FADS2 Antibody - #DF15514

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製品: FADS2 Antibody
カタログ: DF15514
タンパク質の説明: Rabbit polyclonal antibody to FADS2
アプリケーション: IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 52kD(Calculated).
ユニプロット: O95864

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MS Report
HPLC Report
MSDS
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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
FADS2 Antibody detects endogenous levels of FADS2.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

D6D; Delta(6) desaturase; Delta(6) fatty acid desaturase; Delta-6 desaturase; DES 6; DES6; FADS 2; FADS2; FADS2_HUMAN; FADSD 6; FADSD6; Fatty acid desaturase 2; linoleoyl-CoA desaturase (delta-6-desaturase) like 2; LLCDL 2; LLCDL2; SLL0262; TU 13; TU13;

免疫原

免疫原:

A synthesized peptide derived from human FADS2.

Uniprot:

O95864(FADS2_HUMAN) >>Visit HPA database.

遺伝子(ID):
FADS2(9415)
発現特異性:
O95864 FADS2_HUMAN:

Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.

タンパク質配列:
MGKGGNQGEGAAEREVSVPTFSWEEIQKHNLRTDRWLVIDRKVYNITKWSIQHPGGQRVIGHYAGEDATDAFRAFHPDLEFVGKFLKPLLIGELAPEEPSQDHGKNSKITEDFRALRKTAEDMNLFKTNHVFFLLLLAHIIALESIAWFTVFYFGNGWIPTLITAFVLATSQAQAGWLQHDYGHLSVYRKPKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFHKDPDVNMLHVFVLGEWQPIEYGKKKLKYLPYNHQHEYFFLIGPPLLIPMYFQYQIIMTMIVHKNWVDLAWAVSYYIRFFITYIPFYGILGALLFLNFIRFLESHWFVWVTQMNHIVMEIDQEAYRDWFSSQLTATCNVEQSFFNDWFSGHLNFQIEHHLFPTMPRHNLHKIAPLVKSLCAKHGIEYQEKPLLRALLDIIRSLKKSGKLWLDAYLHK

研究背景

機能:

Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively. Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (By similarity). Desaturates palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in a wide array of tissues, highest expression is found in liver followed by brain, lung, heart, and retina. A lower level is found in breast tumor when compared with normal tissues; lowest levels were found in patients with poor prognostic index.

タンパク質ファミリー:

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Belongs to the fatty acid desaturase type 1 family.

研究領域

· Metabolism > Lipid metabolism > alpha-Linolenic acid metabolism.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

参考文献

1). Transcriptome and proteomics conjoint analysis reveal anti-alcoholic liver injury effect of Dianhong Black Tea volatile substances. Food science & nutrition, 2024 (PubMed: 38268900) [IF=3.9]

Application: WB    Species: Rat    Sample:

FIGURE 5 DBTVS decreased the expression of FADS2, CD36, and ACAA1. (a) Representative blots of FADS2, CYP7A1, CD36, ACSL1, ACAA1, and HMGCS2. (b) The protein expression levels of FADS2 between different groups. (c) The protein expression levels of CYP7A1 between different groups. (d) The protein expression levels of CD36 between different groups. (e) The protein expression levels of ACSL1 between different groups. (f) The protein expression levels of ACAA1 between different groups. (g) The protein expression levels of HMGCS2 between different groups. Data were presented as mean ± SD. Multiple groups were tested by one‐way ANOVA followed by Dunnett‐t test for all statistical analyses. *p 

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