製品: ZBTB32 Antibody
カタログ: DF15527
タンパク質の説明: Rabbit polyclonal antibody to ZBTB32
アプリケーション: ELISA(peptide)
反応性: Human, Mouse
分子量: 53kD(Calculated).
ユニプロット: Q9Y2Y4

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製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
ZBTB32 Antibody detects endogenous levels of ZBTB32.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FANCC interacting protein; FANCC-interacting protein; Fanconi anemia zinc finger protein; FAXF; FAZF; OTTHUMP00000045790; repressor of GATA; Rog; Testis zinc finger protein; ZBT32_HUMAN; Zbtb32; ZBTB32 protein; Zinc finger and BTB domain containing 32; Zinc finger and BTB domain-containing protein 32; Zinc finger protein 538; ZNF538;

免疫原

免疫原:

A synthesized peptide derived from human ZBTB32.

Uniprot:
遺伝子(ID):
発現特異性:
Q9Y2Y4 ZBT32_HUMAN:

Predominantly expressed in testis. Some isoforms are ubiquitously expressed.

タンパク質配列:
MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEGISPSTFAQLLNFVYGESVELQPGELRPLQEAARALGVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPERELGDPGEKQKPEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPVGQRGADGKHGVLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWAEAPWLVGGQPALWSILLMPPRYGIPFYHSTPTTGAWQEVWREQRIPLSLNAPKGLWSQNQLASSSPTPGSLPQGPAQLSPGEMEESDQGHTGALATCAGHEDKAGCPPRPHPPPAPPARSRPYACSVCGKRFSLKHQMETHYRVHTGEKPFSCSLCPQRSRDFSAMTKHLRTHGAAPYRCSLCGAGCPSLASMQAHMRGHSPSQLPPGWTIRSTFLYSSSRPSRPSTSPCCPSSSTT

研究背景

機能:

DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).

細胞の位置付け:

Nucleus.
Note: Located in nuclear speckles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in testis. Some isoforms are ubiquitously expressed.

サブユニット構造:

Homodimer (via PTB domain). Interacts with the N-terminal of FANCC. Interacts with ZBTB16. Interacts with GATA3 (By similarity).

タンパク質ファミリー:

The C-terminal zinc finger domain functions as a transcriptional transactivator.

The BTB (POZ) domain possesses repressor activity.

Belongs to the krueppel C2H2-type zinc-finger protein family.

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