CPI17alpha Antibody - #AF6473
(1)
(1)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6473, RRID:AB_2835292.
折りたたみ／展開
17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17kDa PKC potentiated inhibitory protein of PP1; CPI 17 alpha; CPI 17; CPI 17alpha; CPI-17; CPI17; PKC potentiated inhibitory protein of PP1; PKC potentiated inhibitory protein of PP1, 17-KD; PP14A_HUMAN; PPP1INL; Ppp1r14a; Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD; Protein kinase C-potentiated inhibitor protein of 17 kDa; Protein phosphatase 1; Protein phosphatase 1 regulatory (inhibitor) subunit 14A; Protein phosphatase 1 regulatory subunit 14A; Regulatory subunit 14A;
免疫原
- Q96A00 PP14A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96A00 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R5 | Methylation | Uniprot | |
| S12 | Phosphorylation | O43293 (DAPK3) | Uniprot |
| S16 | Phosphorylation | Uniprot | |
| R19 | Methylation | Uniprot | |
| R21 | Methylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| K32 | Methylation | Uniprot | |
| T38 | Phosphorylation | Q05655 (PRKCD) , P17612 (PRKACA) , O75116 (ROCK2) , Q02156 (PRKCE) , O43293 (DAPK3) , Q15139 (PRKD1) , Q13976 (PRKG1) , P17252 (PRKCA) , Q13418 (ILK) , Q05513 (PRKCZ) , Q13153 (PAK1) , P98161 (PKD1) | Uniprot |
| K40 | Methylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S134 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot |
研究背景
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Cytoplasm.
Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.
Belongs to the PP1 inhibitor family.
研究領域
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
参考文献
Application: WB Species: Rat Sample:
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.