p62 Dok Antibody - #AF6478
(1)
(1)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF6478, RRID:AB_2835297.
折りたたみ／展開
Docking protein 1 62kD; Docking protein 1; DOK 1; DOK1; DOK1_HUMAN; Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62;
免疫原
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
- Q99704 DOK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGAVMEGPLFLQSQRFGTKRWRKTWAVLYPASPHGVARLEFFDHKGSSSGGGRGSSRRLDCKVIRLAECVSVAPVTVETPPEPGATAFRLDTAQRSHLLAADAPSSAAWVQTLCRNAFPKGSWTLAPTDNPPKLSALEMLENSLYSPTWEGSQFWVTVQRTEAAERCGLHGSYVLRVEAERLTLLTVGAQSQILEPLLSWPYTLLRRYGRDKVMFSFEAGRRCPSGPGTFTFQTAQGNDIFQAVETAIHRQKAQGKAGQGHDVLRADSHEGEVAEGKLPSPPGPQELLDSPPALYAEPLDSLRIAPCPSQDSLYSDPLDSTSAQAGEGVQRKKPLYWDLYEHAQQQLLKAKLTDPKEDPIYDEPEGLAPVPPQGLYDLPREPKDAWWCQARVKEEGYELPYNPATDDYAVPPPRSTKPLLAPKPQGPAFPEPGTATGSGIKSHNSALYSQVQKSGASGSWDCGLSRVGTDKTGVKSEGST
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q99704 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y30 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| R39 | Methylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| C308 | S-Nitrosylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y337 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| Y362 | Phosphorylation | P00519 (ABL1) , P06213 (INSR) , Q13882 (PTK6) | Uniprot |
| Y377 | Phosphorylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| Y398 | Phosphorylation | P07949 (RET) , P06213 (INSR) | Uniprot |
| Y402 | Phosphorylation | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| Y409 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S443 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S446 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| Y449 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | O14920 (IKBKB) | Uniprot |
| S458 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| T481 | Phosphorylation | Uniprot |
研究背景
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.
Constitutively tyrosine-phosphorylated. Phosphorylated by TEC (By similarity). Phosphorylated by LYN (By similarity). Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. Phosphorylated on tyrosine residues by SRMS.
Cytoplasm. Nucleus.
Cytoplasm>Perinuclear region.
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. Expressed in breast cancer.
Interacts with ABL1 (By similarity). Interacts with RasGAP and INPP5D/SHIP1. Interacts directly with phosphorylated ITGB3. Interacts with SRMS (via the SH2 and SH3 domains).
The PTB domain mediates receptor interaction.
Belongs to the DOK family. Type A subfamily.
研究領域
· Human Diseases > Infectious diseases: Viral > Measles.
参考文献
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