Cox2 Antibody - #AF7003

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製品: Cox2 Antibody
カタログ: AF7003
タンパク質の説明: Rabbit polyclonal antibody to Cox2
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 70kDa; 69kD(Calculated).
ユニプロット: P35354
RRID: AB_2835311

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MSDS
説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Cox2 Antibody detects endogenous levels of total Cox2.
RRID:
AB_2835311
引用形式: Affinity Biosciences Cat# AF7003, RRID:AB_2835311.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

COX 2; COX-2; COX2; Cyclooxygenase 2; Cyclooxygenase 2b; Cyclooxygenase; Cyclooxygenase-2; Cyclooxygenase2; EC 1.14.99.1; fj02a10; Glucocorticoid-regulated inflammatory cyclooxygenase; Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase; GRIPGHS; hCox 2; Macrophage activation-associated marker protein P71/73; OTTHUMP00000033524; PES-2; PGG/HS; PGH synthase 2; PGH2_HUMAN; PGHS 2; PGHS-2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2; ptgs2a; TIS10; TIS10 protein; unp1239; wu:fj02a10;

免疫原

免疫原:

A synthesized peptide derived from human Cox2, corresponding to a region within C-terminal amino acids.

Uniprot:

P35354(PGH2_HUMAN) >>Visit HPA database.

遺伝子(ID):
PTGS2(5743)
タンパク質の説明:
COX-2 Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. Homodimer. Belongs to the prostaglandin G/H synthase family.
タンパク質配列:
MLARALLLCAVLALSHTANPCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAEFNTLYHWHPLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, that regulates phagocytic microglia (By similarity).

PTMs:

S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.

Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.

細胞の位置付け:

Microsome membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the prostaglandin G/H synthase family.

研究領域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

参考文献

1). Efficient Therapy of Inflammatory Bowel Disease (IBD) with Highly Specific and Durable Targeted Ta2C Modified with Chondroitin Sulfate (TACS). Advanced Materials, 2023 (PubMed: 37224059) [IF=27.4]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Figure 7.TACS relieves inflammation and restores intestinal barrier function. B)WB analysis of COX-2 and iNOS proteins expression in RAW264.7 cells with different treatments.
2). Piezo1 Upregulation in Monocyte-Derived Macrophages Impairs Post-Myocardial Infarction Cardiac Repair via Defective Efferocytosis and Enhanced Ferroptosis. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 41214880) [IF=15.1]
3). Disruption of Gut Microbiota-Mediated De Novo NAD+ Synthesis Contributes to the Development of Polycystic Ovary Syndrome. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 41082373) [IF=15.1]

Application: WB    Species: Mouse    Sample:

Figure 6 NAD+ inhibits DHEA-induced ferroptosis in PCOS mice. A,B) Ovarian GSH and MDA levels in NMN- and 3-HAA-treated mice (n = 5). C,D) Representative 4-HNE-stained images of ovarian tissues from the indicated groups of mice (scale bar, 20 µm). E,F) Representative TUNEL-stained images of ovarian tissues from the indicated groups of mice (scale bar, 50 µm). G) Ovarian PTGS2 mRNA expression in the indicated groups of mice (n = 5). H,I) Ovarian PTGS2 and GPX4 protein levels analyzed by western blotting in DHEA-treated mice subjected to NMN and 3-HAA treatments (n = 3). J) Ovarian Fe2+ levels in DHEA-induced PCOS mice treated with NMN and 3-HAA (n = 5). For K,L), KGN cells were pretreated with Fer-1 (2 µM) and NMN (1 mM) for 2 h, followed by treatment with DHEA (20 µM) for 24 h. (K) Cell viability assay (n = 5). (L) PTGS2 and GPX4 protein levels in NMN- and Fer-1-treated KGN cells were analyzed by western blotting (n = 3). M. Prepubertal female mice (21-day-old) were intraperitoneally injected with 3-HAA (200 mg kg−1) and Fer-1 (10 mg kg−1), and subcutaneously injected with DHEA (60 mg kg−1) daily for 21 days. Representative H&E-stained images of ovarian tissues from the indicated groups of mice (scale bar, 200 µm) and analysis of the number of cystic follicles and corpora lutea based on H&E-stained sections (n = 5). Data are expressed as mean ± SD. Statistical significance was determined using one-way ANOVA followed by Tukey's test (A, B, and G-M). *p < 0.05, **p < 0.01, and ***p < 0.001 indicate significance.
4). Combination therapy of PKCζ and COX-2 inhibitors synergistically suppress melanoma metastasis. Journal of Experimental & Clinical Cancer Research, 2017 (PubMed: 28865485) [IF=11.3]

Application: WB    Species: mouse    Sample: B16F10

Fig. 5 The expression of p-PKCζ, p-cofilin and COX-2 after combined treatment of J-4 and Celecoxib. (a) Western blotting images of p-cofilin and COX-2 in B16-F10 cells with various treatments for 24 h. (b) Western blotting images of p-cofilin and COX-2 in A375 cells with various treatments for 24 h. (c) Relative mRNA level of PKCζ and COX-2 determined via RT-PCR. (d) The expression of EMT markers, E-Cadherin and Vimentin, and MMP-2/MMP-9 was affected in B16-F10 and A375 cells after various treatments for 24 h. J-4: 25 μM; Celecoxib: 25 μM. * P < 0.05; ** P < 0.01
5). Ligustilide Suppresses Macrophage-Mediated Intestinal Inflammation and Restores Gut Barrier via EGR1-ADAM17-TNF-α Pathway in Colitis Mice. Research (Washington, D.C.), 2025 (PubMed: 40904624) [IF=11.0]

Application: WB    Species: Mouse    Sample: colon tissue

Fig. 3. Ligustilide alleviates macrophage-mediated intestinal inflammation. (A) The number of white blood cells. (B) The content of hemoglobin. (C) The ratio of monocytes. (D) The ratio of granulocytes. (E) Immunofluorescence observed macrophage infiltration in colon. (F) Western blot detected iNOS and COX-2 expressions. (G and H) The content of IL-1β and IL-6 of colon tissues. (I and J) IL-1β and TNF-α mRNA level of RAW264.7 cell. (K) Western blot detected iNOS and COX-2 expressions of RAW264.7 cell. LL, low dose of ligustilide; LM, medium dose of ligustilide; LH, high dose of ligustilide. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. control group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. model group or LPS group.
6). Thymosin α1 alleviates pulpitis by inhibiting ferroptosis of dental pulp cells. International journal of oral science, 2025 (PubMed: 41087337) [IF=10.8]

Application: WB    Species: Rat    Sample:

Fig. 3 The effect of thymosin α1 on the ferroptosis of dental pulp cells (DPCs). a CCK8 assay: The effect of different concentrations of Tα1 on the growth of DPCs. b The expression of PTGS2, FTL, and GPX4 proteins was evaluated using western blot in four groups of DPCs including Control group, LPS-stimulated DPCs (LPS group), LPS-stimulated DPCs with Tα1 addition (LPS + Tα1 group), and LPS-stimulated DPCs with Ptma gene silenced (LPS + shPTMA group). The statistical analysis of PTGS2 (c), FTL (d), and GPX4 (e) proteins expression in four groups of DPCs. (f) Intracellular Fe2+ levels in DPCs were measured by the FerroOrange fluorescent probe (orange). Cell nuclei were stained with DAPI (blue). A ferroptosis inhibitor, ferrostatin-1, was added into LPS-stimulated DPCs (LPS + Fer-1). Scale bars: 50 μm. g Quantitative analysis of the FerroOrange fluorescent intensity of the four groups of DPCs. Bars represent means ± SD. h Flow cytometry of JC-10 of the four groups. Accumulation of JC-10 aggregates in the mitochondrial matrix can be detected by red fluorescence (Ex/Em: 540/590 nm). At mitochondrial depolarization, JC-10 diffuses out of the mitochondria and returns to its monomeric form which exhibits green fluorescence (Ex/Em: 490/525 nm). i Statistical analysis of JC-10 red/green fluorescence ratio among the four groups. The expression of the inflammatory factor TNF-α (j), IL-1β (k), and IL-6 (l) among the four groups of DPCs. *P 
7). Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biology, 2022 (PubMed: 35500533) [IF=10.7]
8). Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon. Carbohydrate Polymers, 2021 (PubMed: 34119183) [IF=10.7]
9). HAMA-SBMA hydrogel with anti-inflammatory properties delivers cartilage organoids, boosting cartilage regeneration. Journal of nanobiotechnology, 2025 (PubMed: 40448111) [IF=10.2]

Application: WB    Species: Rat    Sample:

Fig. 7 HS600 + CCO promotes integrin α5β1 by activating Frzb, inhibiting inflammatory factors. (A) Immunofluorescence staining of SOX9 and COL2 in the CCO, HS600 + CCO and HS600 + CCO + sh738 groups. (B) Quantitative analysis of Figure A. Chondrogenic capacity is diminished following the knockout of Frzb. (C) Western blot analysis of PRG4, COL2 and SOX9 protein expression in the Control, HS600 + CEP, HS600 + CEP + OP, and HS600 + CEP + sh738 groups. The planar culture group demonstrates that HS and CEP promote the formation of articular cartilage through Frzb. (D) Volcano plot of transcriptomic analysis comparing HS600 + CEP and HS600 + CEP + sh738 groups. (E) GO analysis. Adhesion-related pathways are enriched. (F) Immunofluorescence staining of β1 and α5 in the CCO, HS600 + CCO, and HS600 + CCO + sh738 groups. (G) Quantitative analysis of Figure F. HS + CCO activates Frzb to promote the expression of integrin α5β1. (H) Western blot analysis of β1, β-catenin, PI3K, AKT in the Control, HS600 + CEP, HS600 + CEP + OP, HS600 + CEP + sh738. HS + CEP promotes the expression of integrin β1 and inhibit the expression of β-catenin and PI3K-AKT by activating Frzb. (I) Western blot analysis of β1 and α5 in OP or sh738. Activation of Frzb in BMSCs promotes the expression of integrin α5β1. (J) Immunofluorescence staining of β-catenin and PI3K in the CCO, HS600 + CCO, and HS600 + CCO + sh738 groups. (K) Quantitative analysis of Figure J. HS + CCO activates Frzb to inhibit the expression of β-catenin and PI3K. (L) WB analysis of NF-κB, iNOS, COX2 in the Control, HS600 + CEP, HS600 + CEP + OP, HS600 + CEP + sh738 groups. HS + CEP activates Frzb to inhibit the expression of the inflammatory signaling pathway NF-κB and inflammatory factors. All experiments were repeated three times. ns p > 0.05, *0.01 
10). EphB1 promotes the differentiation and maturation of dendritic cells in non-small cell lung cancer. Cancer letters, 2024 (PubMed: 38070822) [IF=9.1]
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