Cytochrome C Antibody - #AF7004
(6)
(9)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF7004, RRID:AB_2835312.
折りたたみ／展開
CYC; CYC_HUMAN; CYCS; Cytochrome c; Cytochrome c somatic; HCS; THC4;
免疫原
- P99999 CYC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P99999 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Acetylation | Uniprot | |
| K9 | Acetylation | Uniprot | |
| K28 | Sumoylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| T50 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y68 | Phosphorylation | Uniprot | |
| K73 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K74 | Acetylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| T79 | Phosphorylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| K100 | Acetylation | Uniprot | |
| K100 | Methylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K101 | Methylation | Uniprot |
研究背景
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
Mitochondrion intermembrane space.
Note: Loosely associated with the inner membrane.
Belongs to the cytochrome c family.
研究領域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Metabolism > Energy metabolism > Sulfur metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
参考文献
Application: WB Species: Rat Sample: kidney tissues
Application: WB Species: human Sample:
Application: WB Species: human Sample: SW480
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