AFfirm™ XRCC1 Mouse Monoclonal Antibody - #BF8039
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair complementing defective repair in chinese hamster cells 1; X ray repair cross complementing 1; X ray repair cross complementing protein 1; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN;
免疫原
A synthesized peptide derived from human XRCC1, corresponding to a region within the internal amino acids.
Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
- P18887 XRCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTISVVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSGSNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFHSPPDKDEAEAPSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPSYAAATLQASSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLPAPTRTPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPFRSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRMRRRLPSRRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQKPPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQEENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYVTAFNGELEDNMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPHQLYGVVPQA
PTMs - P18887 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K129 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K169 | Acetylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K176 | Sumoylation | Uniprot | |
| S184 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| T198 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| Y211 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S229 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S234 | Phosphorylation | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| S236 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| K247 | Sumoylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K256 | Sumoylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| K260 | Acetylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| K271 | Acetylation | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| T284 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| T296 | O-Glycosylation | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| K298 | Acetylation | Uniprot | |
| R310 | Methylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| K383 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S409 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S410 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S416 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S418 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S421 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K431 | Ubiquitination | Uniprot | |
| T437 | Phosphorylation | Uniprot | |
| T440 | O-Glycosylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| S446 | O-Glycosylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S447 | O-Glycosylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| T453 | O-Glycosylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S475 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S485 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| T488 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) | Uniprot |
| Y515 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S518 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| T519 | Phosphorylation | P68400 (CSNK2A1) , P19784 (CSNK2A2) | Uniprot |
| T523 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) | Uniprot |
| S525 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S578 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K620 | Ubiquitination | Uniprot | |
| Y627 | Phosphorylation | Uniprot |
研究背景
Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated.
Nucleus.
Note: Moves from the nucleoli to the global nuclear chromatin upon DNA damage.
Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.
研究領域
· Genetic Information Processing > Replication and repair > Base excision repair.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.