TIMP1 Antibody - #AF7007

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製品: TIMP1 Antibody
カタログ: AF7007
タンパク質の説明: Rabbit polyclonal antibody to TIMP1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 24kDa; 23kD(Calculated).
ユニプロット: P01033
RRID: AB_2835315

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(85%), Bovine(92%), Horse(92%), Sheep(92%), Dog(85%)
クローナリティ:
Polyclonal
特異性:
TIMP1 Antibody detects endogenous levels of total TIMP1.
RRID:
AB_2835315
引用形式: Affinity Biosciences Cat# AF7007, RRID:AB_2835315.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Clgi; Collagenase inhibitor; Collagenase inhibitor, Human; EPA; EPO; Erythroid Potentiating Activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP-1; Timp1; TIMP1 protein; TIMP1_HUMAN; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor;

免疫原

免疫原:
Uniprot:

P01033(TIMP1_HUMAN) >>Visit HPA database.

遺伝子(ID):
TIMP1(7076)
発現特異性:
P01033 TIMP1_HUMAN:

Detected in rheumatoid synovial fluid (at protein level).

タンパク質の説明:
TIMP1 Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16.
タンパク質配列:
MAPFEPLASGILLLLWLIAPSRACTCVPPHPQTAFCNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYKGFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEEFLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFTKTYTVGCEECTVFPCLSIPCKLQSGTHCLWTDQLLQGSEKGFQSRHLACLPREPGLCTWQSLRSQIA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Pig
85
Dog
85
Rabbit
77
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01033 基板として

Site PTM Type Enzyme
K45 Ubiquitination
K70 Ubiquitination
S178 Phosphorylation
K180 Ubiquitination

研究背景

機能:

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Mediates erythropoiesis in vitro; but, unlike IL3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

PTMs:

The activity of TIMP1 is dependent on the presence of disulfide bonds.

N-glycosylated.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in rheumatoid synovial fluid (at protein level).

サブユニット構造:

Interacts with MMP1, MMP3, MMP10 and MMP13, but has only very low affinity for MMP14. Interacts with CD63; identified in a complex with CD63 and ITGB1.

タンパク質ファミリー:

Belongs to the protease inhibitor I35 (TIMP) family.

研究領域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

参考文献

1). Autophagic-CTSB-inflammasome axis modulates hepatic stellate cells activation in arsenic-induced liver fibrosis. Chemosphere, 2020 (PubMed: 31669990) [IF=8.8]

Application: WB    Species: rat    Sample: HSC-t6 cells

Fig,5| NaAsO2-induced activation of NLRP3 inflammasome and HSCs were mediated by cytoplasmic CTSB. (A-C) HSC-t6 cells were pretreated with or without CA-074 me for 2 h and then treated with NaAsO2 for 24 h. (A) The cytoplasmic and lysosomal CTSB was detected by IF. White arrows indicate positive staining (Images are shown at 20×10 magnification). (B, C) The expression level of NLRP3,caspase-1 p20, IL-1β, Collagen-Ⅰ, a-SMA, MMP-1, and TIMP-1 were determined by Western blot.

Application: WB    Species: rat    Sample: livers

Fig.2 NaAsO2 induced autophagy, activation of NLRP3 inflammasome and 662 hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat livers 663 following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B) 664 The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2. 665 The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2. 667 The protein fraction was analyzed by Western blot. (D) The expression level of 668 Collagen- , α-SMA, MMP-1, and TIMP-1in rat livers following exposure to NaAsO2. 669 The protein fraction was analyzed by Western blot. (E) α-SMA was detected by IF. 670 White arrows indicate positive staining. Values are mean ± SD, and n = 5. *p < 0.05, 671 **p < 0.01 compared with the control group; Scale bar = 100 µm
2). Quercetin induces MGMT+ glioblastoma cells apoptosis via dual inhibition of Wnt3a/β-Catenin and Akt/NF-κB signaling pathways. Phytomedicine, 2023 (PubMed: 37451151) [IF=7.9]
3). Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signaling. Frontiers in Pharmacology, 2023 (PubMed: 37021049) [IF=5.6]
4). Phillygenin Inhibits TGF-β1-induced Hepatic Stellate Cell Activation and Inflammation: Regulation of the Bax/Bcl-2 and Wnt/β-catenin Pathways. Inflammation, 2024 (PubMed: 38393550) [IF=5.1]

Application: WB    Species: Mouse    Sample: mHSC

Fig. 5 PHI inhibited TGF-β1-induced HSC activation in mHSCs and LX-2 cells. b Western blotting analyses of α-SMA, Collagen I, TIMP1, and MMP2 in mHSCs.
5). Transforming Growth Factor Beta 1 and p44/42 Expression in Cardinal Ligament Tissues of Patients with Pelvic Organ Prolapse. MEDICAL SCIENCE MONITOR, 2021 (PubMed: 34176919) [IF=3.1]

Application: IHC    Species: Human    Sample: cardinal ligament tissues

Figure 6 Immunohistochemical staining for MMP9, TIMP1, and caspase 3 in the cardinal ligament. (A–F) Representative images showing that compared with the control group, the expression of MMP9 and caspase 3 was significantly higher and that of TIMP1 was significantly lower in the POP group. Quantitative analysis (G–I). * P<0.05. Magnification: 200× (A1–F1) and 400× (A2–F2). POP – pelvic organ prolapse; TGF-β1 – transforming growth factor beta 1.
6). RNA sequencing reveals the potential mechanism of exercise preconditioning for cerebral ischemia reperfusion injury in rats. Brain and behavior, 2024 (PubMed: 38956886) [IF=3.1]

Application: WB    Species: Rat    Sample:

FIGURE 7 Exercise preconditioning (EP) suppressed TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expressions of the cerebral cortices in middle cerebral artery occlusion (MCAO) rats. At 48 h after cerebral ischemia reperfusion injury (CIRI), TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expression levels of cerebral cortices in each group were evaluated by qPCR (a) and Western blotting (b). # p < .05, ## p < .01 versus Sham. *p < .05, **p < .01 versus EP. Results were presented as mean ± SD. n = 3.
7). Protective Effect of the Total Alkaloid Extract from Bulbus Fritillariae pallidiflorae in a Mouse Model of Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease. International journal of chronic obstructive pulmonary disease, 2024 (PubMed: 38881716) [IF=2.8]
8). Modulation of Bleomycin-induced Oxidative Stress and Pulmonary Fibrosis by Ginkgetin in Mice via AMPK. Current Molecular Pharmacology, 2017 (PubMed: 35249515) [IF=2.7]
9). Forsythiaside A Regulates Activation of Hepatic Stellate Cells by Inhibiting NOX4-Dependent ROS. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35035671)

Application: WB    Species: Rat    Sample: HSCs

Figure 4 FA promoted collagen metabolism. (A-B) The expression of MMP-1 and TIMP-1 mRNA. (C-D) The expression of MMP-1 and TIMP-1 proteins. (E-G) The content of MMP-1 and TIMP-1 in medium supernatant. (H) The content of hydroxyproline. Data of three independent experiments were represented by mean ± S.D. ∗∗∗p <0.001 TGF-β1 vs control; ∗∗p <0.01 TGF-β1 vs control. ###p <0.001 TGF-β1 + FA vs TGF-β1;##p <0.01 TGF-β1 + FA vs TGF-β1; #p <0.05 TGF-β1 + FA vs TGF-β1.
10). Jinlian Xiaodu Decoction Protects against Bleomycin-Induced Pulmonary Fibrosis in Rats. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 35783517)

Application: WB    Species: Rat    Sample: pulmonary tissue

Figure 7 Effect of JXD on the cell apoptosis in pulmonary tissue of BLM-induced PF rats. (a) The apoptotic cells in pulmonary tissues were stained by tunel test kit and observed with a fluorescence microscope (magnification, 200×). (b) The quantitative result of TUNEL staining, n = 6. (c) The representative band of Western blot. (d)–(g) The quantitative result of Western blot, n = 3. Data were expressed as mean ± SD. ##P < 0.01 vs. control; ∗p < 0.05, ∗∗p < 0.01 vs. BLM.
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