AFfirm™ Glutamine Synthetase Mouse Monoclonal Antibody - #BF8094
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
cell proliferation-inducing protein 59; GLNA; GLNA_HUMAN; GLNS; GLUL; Glutamate ammonia ligase; Glutamate decarboxylase; Glutamate--ammonia ligase; glutamine synthase; Glutamine synthetase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43;
免疫原
- P15104 GLNA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
PTMs - P15104 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| K11 | Acetylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| Y180 | Phosphorylation | Uniprot | |
| C183 | S-Nitrosylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| C359 | S-Nitrosylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| K372 | Acetylation | Uniprot | |
| K372 | Ubiquitination | Uniprot |
研究背景
Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine. Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts. Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation. May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ. Plays a role in ribosomal 40S subunit biogenesis.
Palmitoylated; undergoes autopalmitoylation.
Ubiquitinated by ZNRF1.
Cytoplasm>Cytosol. Microsome. Mitochondrion. Cell membrane>Lipid-anchor.
Note: Mainly localizes in the cytosol, with a fraction associated with the cell membrane.
Expressed in endothelial cells.
Decamer; composed of two pentamers. Interacts with PALMD (By similarity). Interacts with RHOJ.
Belongs to the glutamine synthetase family.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Metabolism > Amino acid metabolism > Arginine biosynthesis.
· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.
· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.
· Metabolism > Energy metabolism > Nitrogen metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > GABAergic synapse.
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