AFfirm™ EEF1A2 Mouse Monoclonal Antibody - #BF8103

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製品: EEF1A2 Mouse Monoclonal Antibody
カタログ: BF8103
タンパク質の説明: Mouse monoclonal antibody to EEF1A2
アプリケーション: WB
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 50 kDa; 50kD(Calculated).
ユニプロット: Q05639

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm8103]
特異性:
EEF1A2 Antibody detects endogenous levels of total EEF1A2.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CCS3; eEF1A-1; eEF1A-2; EEF1A1; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-1; EF-1-alpha-2; EF-Tu; EF1A; EF1A1_HUMAN; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 1; Elongation factor 1-alpha 2; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; GRAF 1EF; HS1; Leukocyte receptor cluster member 7; PTI1; Statin-S1;

免疫原

免疫原:
Uniprot:

Q05639(EF1A2_HUMAN) >>Visit HPA database.

遺伝子(ID):
EEF1A2(1917)
発現特異性:
Q05639 EF1A2_HUMAN:

Brain, heart, and skeletal muscle.

タンパク質配列:
MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

PTMs - Q05639 基板として

Site PTM Type Enzyme
S21 Phosphorylation P15056 (BRAF) , P04049 (RAF1)
T22 Phosphorylation
T23 Phosphorylation
T24 Phosphorylation
Y29 Phosphorylation
K30 Acetylation
K30 Methylation
K30 Ubiquitination
K36 Acetylation
K36 Methylation
K36 Ubiquitination
R37 Methylation
K41 Acetylation
K41 Sumoylation
K41 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K51 Methylation
K51 Ubiquitination
S53 Phosphorylation P05771 (PRKCB)
K55 Acetylation
K55 Methylation
K55 Sumoylation
K55 Ubiquitination
Y56 Phosphorylation
K62 Methylation
K62 Ubiquitination
R69 Methylation
K79 Acetylation
K79 Methylation
K84 Methylation
Y85 Phosphorylation
Y86 Phosphorylation
T88 Phosphorylation
T104 Phosphorylation
T106 Phosphorylation
S128 Phosphorylation
K129 Ubiquitination
T133 Phosphorylation
R134 Methylation
Y141 Phosphorylation
T142 Phosphorylation
K146 Acetylation
K146 Ubiquitination
K154 Ubiquitination
S163 Phosphorylation
K165 Acetylation
K165 Methylation
K165 Ubiquitination
R166 Methylation
K172 Ubiquitination
S175 Phosphorylation
Y177 Phosphorylation
K179 Acetylation
S205 Phosphorylation P45983 (MAPK8)
K219 Ubiquitination
T234 Phosphorylation
T239 Phosphorylation
K244 Ubiquitination
Y254 Phosphorylation
K255 Acetylation
K255 Methylation
K255 Ubiquitination
T261 Phosphorylation
R266 Methylation
T269 Phosphorylation
T287 Phosphorylation
S291 Phosphorylation
S300 Phosphorylation
K313 Sumoylation
K313 Ubiquitination
K318 Acetylation
K318 Methylation
K318 Ubiquitination
S358 Phosphorylation P45983 (MAPK8)
S383 Phosphorylation
K392 Ubiquitination
S393 Phosphorylation
R430 Methylation
T432 Phosphorylation
K439 Acetylation
S445 Phosphorylation
T452 Phosphorylation
K453 Ubiquitination

研究背景

機能:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

PTMs:

Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT; dimethylated form is predominant.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Brain, heart, and skeletal muscle.

サブユニット構造:

Monomer.

タンパク質ファミリー:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

研究領域

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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