VEGFB Antibody - #AF7019

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製品: VEGFB Antibody
カタログ: AF7019
タンパク質の説明: Rabbit polyclonal antibody to VEGFB
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep
分子量: 17,21kDa; 22kD(Calculated).
ユニプロット: P49765
RRID: AB_2835323

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(80%), Bovine(80%), Horse(90%), Sheep(80%)
クローナリティ:
Polyclonal
特異性:
VEGFB Antibody detects endogenous levels of total VEGFB.
RRID:
AB_2835323
引用形式: Affinity Biosciences Cat# AF7019, RRID:AB_2835323.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Vascular endothelial growth factor B; Vascular endothelial growth factor-related factor; VEGF B; VEGF related factor; VEGF-B; VEGF-related factor; Vegfb; VEGFB_HUMAN; VEGFL; VRF;

免疫原

免疫原:
Uniprot:

P49765(VEGFB_HUMAN) >>Visit HPA database.

遺伝子(ID):
VEGFB(7423)
発現特異性:
P49765 VEGFB_HUMAN:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

タンパク質の説明:
Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo. Two additional proteins des- ignated VEGF-B and VEGF-C share a significant degree of homology withVEGF.
タンパク質配列:
MSPLLRRLLLAALLQLAPAQAPVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Pig
80
Bovine
80
Sheep
80
Dog
70
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

PTMs:

VEGF-B186 is O-glycosylated.

細胞の位置付け:

Secreted.
Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

サブユニット構造:

Homodimer; disulfide-linked. Can also form heterodimer with VEGF.

タンパク質ファミリー:

Belongs to the PDGF/VEGF growth factor family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

参考文献

1). Astragaloside IV alleviates liver injury in type 2 diabetes due to promotion of AMPK/mTOR‑mediated autophagy. Molecular Medicine Reports, 2021 (PubMed: 33846768) [IF=3.4]

Application: WB    Species: Rat    Sample: kidney tissue

Figure 6. AS-IV activates the suppressed autophagy in the liver of T2DM rats. (A) Representative images of Beclin1, LC3 and P62 (immunohistochemical staining; magnification, ×400). Quantitative analysis of (B) Beclin1, (C) LC3 and (D) P62 were normalized to the normal control group. (E) Protein expression levels of Beclin1, LC3 and P62 in the liver tissues were detected via western blotting. Semi-quantitative analysis of (F) Beclin1, (G) LC3 and (H) P62 was normalised to β-actin. Data are presented as the mean ± SD, n=3. *P<0.05, ***P<0.001 vs. control group; #P<0.05, ##P<0.01, ###P<0.001 vs. model group. Con, Control; Mod, Model; AS-IV, Astragaloside IV (80 mg/kg); Met, Metformin (200 mg/kg).
2). Preventive and inhibitive effects of Yiwei Xiaoyu granules on the development and progression of spasmolytic polypeptide-expressing metaplasia lesions. World Journal of Gastrointestinal Oncology, 2021 (PubMed: 34853647) [IF=3.0]

Application: IHC    Species: Mice    Sample:

Figure 9 The expression of vascular endothelial growth factor-β was restored with Yiwei Xiaoyu granule administration in the spasmolytic polypeptide-expressing metaplasia lesions. A: The expression of vascular endothelial growth factor-β was upregulated with Yiwei Xiaoyu granules administration by immunohistochemistry measurement. B: Statistical differences existed between the control and the model (bP < 0.01). YWXY-H: High dose of Yiwei Xiaoyu Granules; YWXY-L: Low dose of Yiwei Xiaoyu Granules; VEGF: Vascular endothelial growth factor.

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