AFfirm™ PDCD4 Mouse Monoclonal Antibody - #BF8141

(3)  
製品: PDCD4 Mouse Monoclonal Antibody
カタログ: BF8141
タンパク質の説明: Mouse monoclonal antibody to PDCD4
アプリケーション: WB IHC
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 51kDa; 52kD(Calculated).
ユニプロット: Q53EL6

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Monoclonal [AFfirm8141]
特異性:
PDCD4 Antibody detects endogenous levels of total PDCD4.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein;

免疫原

免疫原:
Uniprot:

Q53EL6(PDCD4_HUMAN) >>Visit HPA database.

遺伝子(ID):
PDCD4(27250)
発現特異性:
Q53EL6 PDCD4_HUMAN:

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

タンパク質の説明:
This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.
タンパク質配列:
MDVENEQILNVNPADPDNLSDSLFSGDEENAGTEEIKNEINGNWISASSINEARINAKAKRRLRKNSSRDSGRGDSVSDSGSDALRSGLTVPTSPKGRLLDRRSRSGKGRGLPKKGGAGGKGVWGTPGQVYDVEEVDVKDPNYDDDQENCVYETVVLPLDERAFEKTLTPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY

PTMs - Q53EL6 基板として

Site PTM Type Enzyme
M1 Acetylation
S20 Phosphorylation
S22 Phosphorylation
S25 Phosphorylation
S46 Phosphorylation
K58 Ubiquitination
K60 Ubiquitination
S67 Phosphorylation P31749 (AKT1) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2)
S68 Phosphorylation
S71 Phosphorylation
R73 Methylation
S76 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
S87 Phosphorylation
T90 Phosphorylation
T93 Phosphorylation
S94 Phosphorylation
K96 Ubiquitination
K121 Ubiquitination
T126 Phosphorylation
Y131 Phosphorylation
Y143 Phosphorylation
Y152 Phosphorylation
K166 Ubiquitination
K212 Ubiquitination
K238 Ubiquitination
K242 Ubiquitination
K245 Ubiquitination
T254 Phosphorylation
R267 Methylation
K283 Ubiquitination
K297 Ubiquitination
T299 Phosphorylation
S303 Phosphorylation
K306 Ubiquitination
K311 Ubiquitination
S313 Phosphorylation
S317 Phosphorylation
S323 Phosphorylation
S345 Phosphorylation
K392 Ubiquitination
K402 Ubiquitination
Y405 Phosphorylation
S440 Phosphorylation
S457 Phosphorylation P31749 (AKT1)
K465 Sumoylation
S468 Phosphorylation

研究背景

機能:

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA (By similarity).

PTMs:

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

サブユニット構造:

Interacts (via MI domains) with EIF4A2 (By similarity). Interacts (via MI domains) with EIF4A1 (via N-terminal domain). Heterotrimer with EIF4A1; one molecule of PDCD4 binds two molecules of EIF4A1. Interacts with EIF4G1. May form a complex with EIF4A1 and EIF4G1. The interaction between PDCD4 and EIF4A1 interferes with the interaction between EIF4A1 and EIF4G. When phosphorylated, interacts with BTRC and FBXW11.

タンパク質ファミリー:

Binds EIF4A1 via both MI domains.

Belongs to the PDCD4 family.

研究領域

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.