AFfirm™ ADRB2 Mouse Monoclonal Antibody - #BF8223
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
ADRB2; ADRB2_HUMAN; ADRB2R; ADRBR; Adrenergic beta 2 receptor surface; Adrenoceptor beta 2 surface; B2AR; BAR; beta 2 adrenoceptor; Beta 2 adrenoreceptor; Beta-2 adrenergic receptor; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; Catecholamine receptor; OTTHUMP00000160386;
免疫原
- P07550 ADRB2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGQPGNGSAFLLAPNGSHAPDHDVTQERDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL
PTMs - P07550 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Ubiquitination | Uniprot | ||
| T68 | Phosphorylation | Uniprot | |
| Y132 | Phosphorylation | P08069 (IGF1R) , P06213 (INSR) | Uniprot |
| Y141 | Phosphorylation | P06213 (INSR) , P08069 (IGF1R) | Uniprot |
| Y219 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S262 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| Y308 | Phosphorylation | Uniprot | |
| Y326 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | P31749 (AKT1) , P17612 (PRKACA) , Q05655 (PRKCD) | Uniprot |
| S346 | Phosphorylation | P17612 (PRKACA) , Q05655 (PRKCD) , P31749 (AKT1) | Uniprot |
| Y350 | Phosphorylation | P06213 (INSR) | Uniprot |
| Y354 | Phosphorylation | P06213 (INSR) | Uniprot |
| S355 | Phosphorylation | P43250 (GRK6) , P35626 (GRK3) , P25098 (GRK2) , P34947 (GRK5) | Uniprot |
| S356 | Phosphorylation | P43250 (GRK6) , P25098 (GRK2) , P35626 (GRK3) , P34947 (GRK5) | Uniprot |
| T360 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| T384 | Phosphorylation | P25098 (GRK2) , P34947 (GRK5) | Uniprot |
| T393 | Phosphorylation | P34947 (GRK5) | Uniprot |
| S396 | Phosphorylation | P25098 (GRK2) , P34947 (GRK5) | Uniprot |
| S401 | Phosphorylation | P25098 (GRK2) , P34947 (GRK5) | Uniprot |
| S407 | Phosphorylation | P25098 (GRK2) , P34947 (GRK5) | Uniprot |
| T408 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | P25098 (GRK2) , P34947 (GRK5) | Uniprot |
研究背景
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.
Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.
Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.
Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.
Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.
Cell membrane>Multi-pass membrane protein. Early endosome.
Note: Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325).
Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33. Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation. Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with CNIH4. Interacts with ARRDC3.
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
研究領域
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
· Organismal Systems > Endocrine system > Renin secretion.
· Organismal Systems > Digestive system > Salivary secretion.
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