Phospho-RPS6 (Ser235) Antibody-Fluor647 - #AF3354-F647
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
40S ribosomal protein S6; Air8; NP33; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; rps6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein;
免疫原
A synthesized peptide derived from human RPS6 around the phosphorylation site of Ser235.
- P62753 RS6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK
PTMs - P62753 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K2 | Methylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| C12 | S-Nitrosylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| R22 | Methylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| K30 | Acetylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K79 | Acetylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| R154 | Methylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K218 | Acetylation | Uniprot | |
| S235 | Phosphorylation | Q13177 (PAK2) , P51812 (RPS6KA3) , P53355 (DAPK1) , Q05655 (PRKCD) , P23443 (RPS6KB1) , Q96RG2 (PASK) , Q15418 (RPS6KA1) | Uniprot |
| S236 | Phosphorylation | Q05655 (PRKCD) , P23443 (RPS6KB1) , P51812 (RPS6KA3) , Q13177 (PAK2) , Q15418 (RPS6KA1) , Q96RG2 (PASK) | Uniprot |
| S240 | Phosphorylation | Q13177 (PAK2) , P23443 (RPS6KB1) , Q15418 (RPS6KA1) | Uniprot |
| T241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| S244 | Phosphorylation | Q15418 (RPS6KA1) , P23443 (RPS6KB1) | Uniprot |
| S246 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | P23443 (RPS6KB1) | Uniprot |
研究背景
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Specifically hydroxylated (with R stereochemistry) at C-3 of Arg-137 by KDM8.
Belongs to the eukaryotic ribosomal protein eS6 family.
研究領域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Genetic Information Processing > Translation > Ribosome.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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