LC3A Antibody - #AF4007

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製品: LC3A Antibody
カタログ: AF4007
タンパク質の説明: Rabbit polyclonal antibody to LC3A
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 19kd; 14kD(Calculated).
ユニプロット: Q9H492
RRID: AB_2835334

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-1:5000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
MAP1LC3A Antibody detects endogenous levels of total MAP1LC3A.
RRID:
AB_2835334
引用形式: Affinity Biosciences Cat# AF4007, RRID:AB_2835334.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Map1lc3a; Microtubule associated proteins 1A/1B light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated proteins 1A and 1B, light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human MAP1LC3A, corresponding to a region within the internal amino acids.

Uniprot:

Q9H492(MLP3A_HUMAN) >>Visit HPA database.

遺伝子(ID):
MAP1LC3A(84557)
発現特異性:
Q9H492 MLP3A_HUMAN:

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

タンパク質の説明:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
タンパク質配列:
MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, paticipates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.

PTMs:

The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.

Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Endomembrane system>Lipid-anchor. Cytoplasmic vesicle>Autophagosome membrane>Lipid-anchor. Cytoplasmic vesicle>Autophagosome.
Note: LC3-II binds to the autophagic membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

タンパク質ファミリー:

Belongs to the ATG8 family.

研究領域

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

参考文献

1). Zinc ions facilitate metabolic bioenergetic recovery post spinal cord injury by activating microglial mitophagy through the STAT3-FOXO3a-SOD2 pathway. Free radical biology & medicine, 2024 (PubMed: 39613048) [IF=7.1]
2). CH25H Promotes Autophagy and Regulates the Malignant Progression of Laryngeal Squamous Cell Carcinoma Through the PI3K-AKT Pathway. Cancer medicine, 2024 (PubMed: 39428922) [IF=2.9]

Application: WB    Species: Human    Sample: LSCC Cells

FIGURE 5. CH25H inhibited the proliferation of LSCC cells (A) After overexpressing CH25H in TU‐686 and knocking down CH25H in AMC‐HN‐8, the expression of CH25H was detected; (B) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the cell viability was detected; (C) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the cell colony formation was detected; (D) The cell proliferation of each group was displayed by the bar chart; (E) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the cell apoptosis was detected; (F) The apoptosis level of each group was displayed by the bar chart; (G) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the migration was detected; (H) After TU‐686 were overexpressed with CH25H and AMC‐HN‐8 were knocked down with CH25H, the invasion was detected; (I) After overexpressing CH25H in TU‐686 and knocking down CH25H in AMC‐HN‐8, the expression of autophagy‐related proteins was detected by WB (**p 
3). CircPAN3 contributes to drug resistance in acute myeloid leukemia through regulation of autophagy. LEUKEMIA RESEARCH, 2019 (PubMed: 31401408) [IF=2.1]

Application: WB    Species: human    Sample: THP-1, THP-1/ADM, K562 and K562/ADM cells

Fig. 3. |The levels of autophagy markers in drug-sensitive and -resistant AML cells. (A) Autophagy related proteins LC3-II/LC3-I, Beclin-1, and P62 were detected and quantified by Western blot in THP-1, THP-1/ADM, K562 and K562/ADM cells.

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