製品: eEF2K Mouse Monoclonal Antibody
カタログ: BF8287
タンパク質の説明: Mouse monoclonal antibody to eEF2K
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 82kDa; 82kD(Calculated).
ユニプロット: O00418

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Monoclonal [AFfirm8287]
特異性:
eEF2K Mouse Monoclonal Antibody detects endogenous levels of total eEF2K
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

C86191; Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; EC 2.7.11.20; eEF 2 kinase; eEF 2K; eEF-2 kinase; eEF-2K; Eef2k; EEF2K protein; EF2K_HUMAN; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; fa04b08; HSU93850; kinase eEF2K; MGC45041; SMEF2K; wu:fa04b08; zgc:100973;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
タンパク質配列:
MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYYSNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAVTGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDVYFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNLGVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSPQVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDNMASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGRVCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFCEKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQDEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAWAQMEE

PTMs - O00418 基板として

Site PTM Type Enzyme
A2 Acetylation
S18 Phosphorylation
S27 Phosphorylation
S31 Phosphorylation
Y37 Phosphorylation
K58 Ubiquitination
Y59 Phosphorylation
Y60 Phosphorylation
S61 Phosphorylation O00418 (EEF2K)
K65 Ubiquitination
S66 Phosphorylation O00418 (EEF2K)
Y69 Phosphorylation
S70 Phosphorylation
S71 Phosphorylation
S72 Phosphorylation P42345 (MTOR)
S74 Phosphorylation P42345 (MTOR)
S78 Phosphorylation O00418 (EEF2K) , P54646 (PRKAA2) , Q13131 (PRKAA1) , Q96QT4 (TRPM7)
K82 Ubiquitination
K86 Ubiquitination
K93 Ubiquitination
K132 Ubiquitination
S135 Phosphorylation
K151 Ubiquitination
K162 Ubiquitination
K170 Ubiquitination
R178 Methylation
K202 Ubiquitination
K223 Ubiquitination
K235 Ubiquitination
K238 Ubiquitination
S241 Phosphorylation
S243 Phosphorylation
T340 Phosphorylation
K341 Ubiquitination
S345 Phosphorylation
K347 Ubiquitination
T348 Phosphorylation O00418 (EEF2K)
T353 Phosphorylation O00418 (EEF2K)
S359 Phosphorylation P06493 (CDK1) , O15264 (MAPK13)
T364 Phosphorylation
S366 Phosphorylation Q15418 (RPS6KA1) , Q13131 (PRKAA1) , P17612 (PRKACA) , P54646 (PRKAA2) , O00418 (EEF2K) , P23443 (RPS6KB1) , Q9UBS0 (RPS6KB2)
S377 Phosphorylation Q16644 (MAPKAPK3) , P49137 (MAPKAPK2) , Q8IW41 (MAPKAPK5)
S380 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation P45984 (MAPK9) , P45983 (MAPK8) , P53778 (MAPK12) , Q15759 (MAPK11) , O15264 (MAPK13) , O00418 (EEF2K) , Q16539 (MAPK14)
S398 Phosphorylation Q13131 (PRKAA1) , P54646 (PRKAA2)
S399 Phosphorylation
T401 Phosphorylation
S416 Phosphorylation
S435 Phosphorylation
S438 Phosphorylation
S441 Phosphorylation O00418 (EEF2K)
Y443 Phosphorylation
S445 Phosphorylation O00418 (EEF2K)
K447 Ubiquitination
S462 Phosphorylation
K467 Sumoylation
Y468 Phosphorylation
S470 Phosphorylation
S474 Phosphorylation O00418 (EEF2K)
S477 Phosphorylation
S478 Phosphorylation
K485 Ubiquitination
S491 Phosphorylation O00418 (EEF2K)
S492 Phosphorylation
S500 Phosphorylation O00418 (EEF2K) , P17612 (PRKACA)
K516 Ubiquitination
K517 Ubiquitination
K520 Ubiquitination
S521 Phosphorylation
K596 Ubiquitination
Y600 Phosphorylation
K603 Ubiquitination
S627 Phosphorylation
K684 Ubiquitination
K705 Ubiquitination
K715 Ubiquitination

PTMs - O00418 酵素として

Substrate Site Source
O00418 (EEF2K) S61 Uniprot
O00418 (EEF2K) S66 Uniprot
O00418 (EEF2K) S78 Uniprot
O00418 (EEF2K) T348 Uniprot
O00418 (EEF2K) T353 Uniprot
O00418 (EEF2K) S366 Uniprot
O00418 (EEF2K) S396 Uniprot
O00418 (EEF2K) S441 Uniprot
O00418 (EEF2K) S445 Uniprot
O00418 (EEF2K) S474 Uniprot
O00418 (EEF2K) S491 Uniprot
O00418 (EEF2K) S500 Uniprot
P13639 (EEF2) T54 Uniprot
P13639 (EEF2) T57 Uniprot
P13639 (EEF2) T59 Uniprot

研究背景

機能:

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

PTMs:

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.

サブユニット構造:

Monomer or homodimer (Probable). Interacts with Calmodulin/CALM1; this interaction is strictly required for phosphorylation activity.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. Alpha-type protein kinase family.

研究領域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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