AFfirm™ TRIM32 Mouse Monoclonal Antibody - #BF8323
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A;
免疫原
- Q13049 TRI32_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP
PTMs - Q13049 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S7 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| S651 | Phosphorylation | Uniprot |
研究背景
Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.
Ubiquitinated.
Cytoplasm.
Note: Localized in cytoplasmic bodies, often located around the nucleus.
Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.
Interacts with DTNBP1. It self-associates.
Belongs to the TRIM/RBCC family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.