AFfirm™ EIF6 Mouse Monoclonal Antibody - #BF8325

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製品: EIF6 Mouse Monoclonal Antibody
カタログ: BF8325
タンパク質の説明: Mouse monoclonal antibody to EIF6
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 27kDa; 27kD(Calculated).
ユニプロット: P56537

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Monoclonal [AFfirm8325]
特異性:
EIF6 Mouse Monoclonal Antibody detects endogenous levels of total EIF6
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

b(2)gcn; B(2)GCN homolog; B4 integrin interactor; Binding protein of beta-4 integrin; CAB; eIF-6; EIF3A; EIF6; Eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; IF6_HUMAN; ITGB4BP; OK/SW-cl.27; p27 beta 4 integrin binding protein; p27(BBP); p27BBP; RP4-614O4.1;

免疫原

免疫原:
Uniprot:

P56537(IF6_HUMAN) >>Visit HPA database.

遺伝子(ID):
EIF6(3692)
発現特異性:
P56537 IF6_HUMAN:

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

タンパク質の説明:
Eukaryotic initiation factor 6 (eIF6) is reqiured for the 60S ribosomal subunit assembly in the nucleolus (1). In the cytoplasm, this protein is bound to 60S ribosome subunits and prevents them from joining 40S ribosome subunits to form 80S ribosomes (2). eIF6 is also shown to associate with the RNA-induced silencing complex (RISC) (3). Deletion of eIF6 abolishes the miRNA-mediated gene silencing (3). eIF6 may play its essential role in miRNA-mediated silencing by inhibiting translation initiation or ribosome recycling (3).
タンパク質配列:
MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

PTMs - P56537 基板として

Site PTM Type Enzyme
S6 Phosphorylation
C11 S-Nitrosylation
C15 S-Nitrosylation
R85 Methylation
R95 Methylation
Y113 Phosphorylation
S150 Phosphorylation
S174 Phosphorylation P48729 (CSNK1A1)
S175 Phosphorylation P48729 (CSNK1A1)
T185 Phosphorylation
K223 Acetylation
S230 Phosphorylation
T231 Phosphorylation
T234 Phosphorylation
S235 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB)
S239 Phosphorylation
S243 Phosphorylation
T245 Phosphorylation

研究背景

機能:

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).

PTMs:

Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.

細胞の位置付け:

Cytoplasm. Nucleus>Nucleolus.
Note: Shuttles between cytoplasm and nucleus/nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).

サブユニット構造:

Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).

タンパク質ファミリー:

Belongs to the eIF-6 family.

研究領域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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