AFfirm™ SHP2 Mouse Monoclonal Antibody - #BF8332
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
BPTP3; CFC; JMML; METCDS; MGC14433; NS1; OTTHUMP00000166107; OTTHUMP00000166108; Protein tyrosine phosphatase 2; Protein tyrosine phosphatase 2C; Protein tyrosine phosphatase non receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11_HUMAN; PTP-1D; PTP-2C; PTP1D; PTP2C; PTPN11; SAP2; SH-PTP2; SH-PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHP 2; SHP-2; Shp2; SHPTP2; SHPTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11;
免疫原
- Q06124 PTN11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLADQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQKSFR
PTMs - Q06124 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| Y63 | Phosphorylation | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| K178 | Acetylation | Uniprot | |
| Y179 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K198 | Acetylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| Y263 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| Y279 | Phosphorylation | Uniprot | |
| K280 | Acetylation | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| Y327 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| K486 | Acetylation | Uniprot | |
| Y515 | Phosphorylation | Uniprot | |
| Y519 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| Y546 | Phosphorylation | P08922 (ROS1) , P09619 (PDGFRB) , P06241 (FYN) , Q9UM73 (ALK) | Uniprot |
| T547 | Phosphorylation | Uniprot | |
| Y551 | Phosphorylation | Uniprot | |
| S552 | Phosphorylation | Uniprot | |
| T557 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | Uniprot | |
| T570 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | P17252 (PRKCA) , P05771 (PRKCB) , P24723 (PRKCH) | Uniprot |
| Y584 | Phosphorylation | P08922 (ROS1) , P00519 (ABL1) , Q9UM73 (ALK) , P42684 (ABL2) | Uniprot |
| K594 | Ubiquitination | Uniprot | |
| S595 | Phosphorylation | P05771-2 (PRKCB) , P24723 (PRKCH) , P17252 (PRKCA) | Uniprot |
PTMs - Q06124 酵素として
研究背景
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.
Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.
Cytoplasm. Nucleus.
Widely expressed, with highest levels in heart, brain, and skeletal muscle.
Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Interacts with FLT1 (tyrosine-phosphorylated), FLT3 (tyrosine-phosphorylated), FLT4 (tyrosine-phosphorylated), KIT and GRB2. Interacts with PDGFRA (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL4, FCRL6 and ANKHD1. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts with GAB2. Interacts with TERT; the interaction retains TERT in the nucleus. Interacts with PECAM1 and FER. Interacts with EPHA2 (activated); participates in PTK2/FAK1 dephosphorylation in EPHA2 downstream signaling. Interacts with ROS1; mediates PTPN11 phosphorylation. Interacts with PDGFRB (tyrosine phosphorylated); this interaction increases the PTPN11 phosphatase activity. Interacts with GAREM1 isoform 1 (tyrosine phosphorylated); the interaction increases MAPK/ERK activity and does not affect the GRB2/SOS complex formation. Interacts with CDC73. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with SIGLEC10 (By similarity). Interacts with FCRL3 (via phosphorylated ITIM motifs).
The SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
研究領域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
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