製品: GABBR2 Mouse Monoclonal Antibody
カタログ: BF8361
タンパク質の説明: Mouse monoclonal antibody to GABBR2
アプリケーション: IF/ICC
反応性: Human
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 106kDa; 106kD(Calculated).
ユニプロット: O75899

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製品説明

ソース:
Mouse
アプリケーション:
IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm8361]
特異性:
GABBR2 Mouse Monoclonal Antibody detects endogenous levels of total GABBR2.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

BcDNA:GH07312; CG6706; CT20836; D Gaba2; FLJ36928; G protein coupled receptor 51; G-protein coupled receptor 51; GAB B R2; GABA B R2; GABA B receptor 2; GABA-B receptor 2; GABA-B-R2; GABA-BR2; GABAB R2; GABABR 2; GABABR2; GABB R2; GABBR 2; Gabbr2; GABR2_HUMAN; Gamma aminobutyric acid B receptor 2; Gamma aminobutyric acid GABA B receptor 2; Gamma aminobutyric acid type B receptor subunit 2; Gamma-aminobutyric acid type B receptor subunit 2; Gb 2; Gb2; GH07312; GPR 51; GPR51; GPRC 3B; GPRC3B; HG 20; HG20; HRIHFB2099; Metabotropic GABA B receptor subtype 2; OTTHUMP00000021776; OTTHUMP00000063797; R2 SUBUNIT;

免疫原

免疫原:

A synthesized peptide derived from human GABBR2 around the site of Ser884.

Uniprot:
遺伝子(ID):
発現特異性:
O75899 GABR2_HUMAN:

Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla (PubMed:10087195, PubMed:10328880, PubMed:10727622, PubMed:9872744). Weakly expressed in heart, testis and skeletal muscle (PubMed:10087195, PubMed:10727622).

タンパク質配列:
MASPRSSGQPGPPPPPPPPPARLLLLLLLPLLLPLAPGAWGWARGAPRPPPSSPPLSIMGLMPLTKEVAKGSIGRGVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDAIKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDNAVNPAILKLLKHYQWKRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSVKKLKGNDVRIILGQFDQNMAAKVFCCAYEENMYGSKYQWIIPGWYEPSWWEQVHTEANSSRCLRKNLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQYEREYNNKRSGVGPSKFHGYAYDGIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGRIILNAMNETNFFGVTGQVVFRNGERMGTIKFTQFQDSREVKVGEYNAVADTLEIINDTIRFQGSEPPKDKTIILEQLRKISLPLYSILSALTILGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLDGSFVSEKTFETLCTVRTWILTVGYTTAFGAMFAKTWRVHAIFKNVKMKKKIIKDQKLLVIVGGMLLIDLCILICWQAVDPLRRTVEKYSMEPDPAGRDISIRPLLEHCENTHMTIWLGIVYAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVGIMCIIGAAVSFLTRDQPNVQFCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSVNQASTSRLEGLQSENHRLRMKITELDKDLEEVTMQLQDTPEKTTYIKQNHYQELNDILNLGNFTESTDGGKAILKNHLDQNPQLQWNTTEPSRTCKDPIEDINSPEHIQRRLSLQLPILHHAYLPSIGGVDASCVSPCVSPTASPRHRHVPPSFRVMVSGL

PTMs - O75899 基板として

Site PTM Type Enzyme
S57 Phosphorylation
N90 N-Glycosylation
T200 Phosphorylation
T202 Phosphorylation
S379 Phosphorylation
N389 N-Glycosylation
N404 N-Glycosylation
S434 Phosphorylation
N453 N-Glycosylation
K583 Acetylation
K589 Acetylation
K768 Ubiquitination
K772 Ubiquitination
K801 Ubiquitination
T813 Phosphorylation
T819 Phosphorylation
S884 Phosphorylation
S893 Phosphorylation P17612 (PRKACA)
S924 Phosphorylation

研究背景

機能:

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable).

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein.
Note: Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. In contrast, GABBR2 does not depend on GABBR1 for transport to the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.

サブユニット構造:

Heterodimer of GABBR1 and GABBR2. Homodimers may form, but are inactive. Interacts (via C-terminus) with ATF4 (via leucine zipper domain) (By similarity).

タンパク質ファミリー:

Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABBR1.

Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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