AFfirm™ SGTA Mouse Monoclonal Antibody - #BF8384
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Alpha SGT; Alpha-SGT; AlphaSGT; hSGT; SGT; Sgt1; SGTA; SGTA_HUMAN; Small glutamine rich protein with tetratricopeptide repeats 1; Small glutamine rich tetratricopeptide repeat containing protein alpha; Small glutamine rich tetratricopeptide repeat TPR containing alpha; Small glutamine rich tetratricopeptide repeat-containing protein; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; Stg; UBP; Vpu binding protein; Vpu-binding protein;
免疫原
- O43765 SGTA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE
PTMs - O43765 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Acetylation | Uniprot | |
| Y9 | Phosphorylation | Uniprot | |
| R76 | Methylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| C129 | S-Nitrosylation | Uniprot | |
| K137 | Acetylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| C148 | S-Nitrosylation | Uniprot | |
| C153 | S-Nitrosylation | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K160 | Acetylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot |
研究背景
Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the proteasome when targeting fails. Functions in tail-anchored/type II transmembrane proteins membrane insertion constituting with ASNA1 and the BAG6 complex a targeting module. Functions upstream of the BAG6 complex and ASNA1, binding more rapidly the transmembrane domain of newly synthesized proteins. It is also involved in the regulation of the endoplasmic reticulum-associated misfolded protein catabolic process via its interaction with BAG6: collaborates with the BAG6 complex to maintain hydrophobic substrates in non-ubiquitinated states. Competes with RNF126 for interaction with BAG6, preventing the ubiquitination of client proteins associated with the BAG6 complex. Binds directly to HSC70 and HSP70 and regulates their ATPase activity.
(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection via interaction with DNAJB12, DNAJB14 and HSPA8/Hsc70.
Cytoplasm. Nucleus.
Note: Co-localizes with HSP90AB1 in the cytoplasm. Increased nuclear accumulation seen during cell apoptosis.
Ubiquitous.
Homodimer. Homooligomer (By similarity). Interacts with DNAJC5 and DNAJC5B. Interacts (via TPR repeats) with HSP90AA1. Interacts (via Gln-rich region) with SLC2A1. Interacts with HSP90AB1. Interacts (via TPR repeats) with HSPA8/Hsc70; the interaction is direct. Interacts with BAG6 (via ubiquitin-like domain); interaction prevents interaction between BAG6 and RNF126. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.
(Microbial infection) Interacts with Vpu and Gag from HIV-1.
(Microbial infection) Interacts with SARS-CoV accessory protein 7a.
The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.
Belongs to the SGT family.
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