AFfirm™ Cyclin F Mouse Monoclonal Antibody - #BF8297
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Ccnf; CCNF_HUMAN; Cyclin-F; F box only protein 1; F-box only protein 1; FBX1; FBXO1; G2 mitotic specific cyclin F; G2/mitotic specific cyclin F;
免疫原
A synthesized peptide derived from human Cyclin F, corresponding to a region within C-terminal amino acids.
- P41002 CCNF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSGGVVHCRCAKCFCYPTKRRIRRRPRNLTILSLPEDVLFHILKWLSVEDILAVRAVHSQLKDLVDNHASVWACASFQELWPSPGNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEARAEVNGLKASRFFSLAERLNVGAAPFIWLFIRPPWSVSGSCCKAVVHESLRAECQLQRTHKASILHCLGRVLSLFEDEEKQQQAHDLFEEAAHQGCLTSSYLLWESDRRTDVSDPGRCLHSFRKLRDYAAKGCWEAQLSLAKACANANQLGLEVRASSEIVCQLFQASQAVSKQQVFSVQKGLNDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRYRLQLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRMMGEIVSALEGKIRVPTVVDYKEVLLTLVPVELRTQHLCSFLCELSLLHTSLSAYAPARLAAAALLLARLTHGQTQPWTTQLWDLTGFSYEDLIPCVLSLHKKCFHDDAPKDYRQVSLTAVKQRFEDKRYGEISQEEVLSYSQLCAALGVTQDSPDPPTFLSTGEIHAFLSSPSGRRTKRKRENSLQEDRGSFVTTPTAELSSQEETLLGSFLDWSLDCCSGYEGDQESEGEKEGDVTAPSGILDVTVVYLNPEQHCCQESSDEEACPEDKGPQDPQALALDTQIPATPGPKPLVRTSREPGKDVTTSGYSSVSTASPTSSVDGGLGALPQPTSVLSLDSDSHTQPCHHQARKSCLQCRPPSPPESSVPQQQVKRINLCIHSEEEDMNLGLVRL
PTMs - P41002 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K514 | Ubiquitination | Uniprot | |
| S577 | Phosphorylation | Uniprot | |
| T588 | Phosphorylation | Uniprot | |
| T590 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| T680 | Phosphorylation | Uniprot | |
| K684 | Ubiquitination | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S774 | Phosphorylation | Uniprot |
研究背景
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.
Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence.
Phosphorylated just before cells enter into mitosis.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus.
Widely expressed.
Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this interaction leads to CCP110 ubiquitination and degradation via the proteasome pathway.
The nuclear localization signals mediate the localization to the nucleus and are required for CCNB1 localization to the nucleus.
Belongs to the cyclin family. Cyclin AB subfamily.
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