AFfirm™ KSR2 Mouse Monoclonal Antibody - #BF8389
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
FLJ25965; hKSR 2; hKSR2; Kinase suppressor of Ras 2; KSR 2; Ksr2; KSR2_HUMAN;
免疫原
- Q6VAB6 KSR2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRNVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVPSKCVQHYCHTSPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRKKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRVDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDPARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEVHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL
PTMs - Q6VAB6 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S34 | Phosphorylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| T260 | Phosphorylation | Uniprot | |
| T264 | Phosphorylation | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| T270 | Phosphorylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| K281 | Acetylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S399 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| T497 | Phosphorylation | Uniprot | |
| Y566 | Phosphorylation | Uniprot | |
| Y794 | Phosphorylation | Uniprot | |
| T802 | Phosphorylation | Uniprot | |
| S808 | Phosphorylation | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| S939 | Phosphorylation | Uniprot |
研究背景
Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1/MEK1-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1. Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production.
Phosphorylated on Ser-474 by MARK3.
Cytoplasm. Membrane>Peripheral membrane protein.
Mainly expressed in brain and kidney.
Heterodimerizes (via N-terminus) with BRAF (via N-terminus) in a MAP2K1/MEK1-dependent manner. Interacts with BRAF; this increases the low intrinsic protein kinase activity of KSR2. Interacts with MAP2K1, forming a heterodimer that can dimerize to form a heterotetramer. Interacts with MAP3K8, MAPK, RAS and RAF.
The protein kinase domain is predicted to be catalytically inactive and seems to have very low intrinsic kinase activity. This low kinase activity can be increased by interaction with BRAF.
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
研究領域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
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