AFfirm™ Caspase 2 Mouse Monoclonal Antibody - #BF8401
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
CASP 2; CASP-2; Casp2; CASP2_HUMAN; Caspase 2; Caspase 2 apoptosis related cysteine peptidase; Caspase-2 subunit p12; Caspase2; ICH 1; ICH 1 protease; ICH 1L; ICH1; ICH1 protease; ICH1L; NEDD-2; NEDD2; Neural precursor cell expressed developmentally down-regulated protein 2; PPP1R57; Protease ICH-1; Protein phosphatase 1 regulatory subunit 57;
免疫原
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
- P42575 CASP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPSAGSWSTFQHKELMAADRGRRILGVCGMHPHHQETLKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVGSFSQNVELLNLLPKRGPQAFDAFCEALRETKQGHLEDMLLTTLSGLQHVLPPLSCDYDLSLPFPVCESCPLYKKLRLSTDTVEHSLDNKDGPVCLQVKPCTPEFYQTHFQLAYRLQSRPRGLALVLSNVHFTGEKELEFRSGGDVDHSTLVTLFKLLGYDVHVLCDQTAQEMQEKLQNFAQLPAHRVTDSCIVALLSHGVEGAIYGVDGKLLQLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHAGSPGCEESDAGKEKLPKMRLPTRSDMICGYACLKGTAAMRNTKRGSWYIEALAQVFSERACDMHVADMLVKVNALIKDREGYAPGTEFHRCKEMSEYCSTLCRHLYLFPGHPPT
PTMs - P42575 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| R22 | Methylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K77 | Sumoylation | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| R107 | Methylation | Uniprot | |
| S139 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| Y151 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S157 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| T160 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| T374 | Phosphorylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot |
研究背景
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress.
The mature protease can process its own propeptide, but not that of other caspases.
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Forms a complex named the PIDDosome with PIDD1 and CRADD. Interacts with NOL3 (via CARD domain); inhibits CASP2 activity in a phosphorylation-dependent manner.
The CARD domain mediates a direct interaction with CRADD.
Belongs to the peptidase C14A family.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
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