製品: FOXM1 Mouse Monoclonal Antibody
カタログ: BF8405
タンパク質の説明: Mouse monoclonal antibody to FOXM1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Rabbit, Dog
分子量: 84kD, 100-130kD; 84kD(Calculated).
ユニプロット: Q08050

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Monoclonal [AFfirm8405]
特異性:
FOXM1 Mouse Monoclonal Antibody detects endogenous levels of total FOXM1
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

FKHL16; Forkhead box M1; Forkhead box protein M1; forkhead like 16; Forkhead-related protein FKHL16; FOX M1; Foxm1; FOXM1_HUMAN; FOXM1B; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11; HNF-3/fork-head homolog 11; HNF3; INS1; M phase phosphoprotein 2; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells; Winged-helix factor from INS1 cells;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q08050 FOXM1_HUMAN:

Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.

タンパク質配列:
MKTSPRRPLILKRRRLPLPVQNAPSETSEEEPKRSPAQQESNQAEASKEVAESNSCKFPAGIKIINHPTMPNTQVVAIPNNANIHSIITALTAKGKESGSSGPNKFILISCGGAPTQPPGLRPQTQTSYDAKRTEVTLETLGPKPAARDVNLPRPPGALCEQKRETCADGEAAGCTINNSLSNIQWLRKMSSDGLGSRSIKQEMEEKENCHLEQRQVKVEEPSRPSASWQNSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKPLDPGSPQLPEHLESQQKRPNPELRRNMTIKTELPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPKVLLAEEGIAPLSSAGPGKEEKLLFGEGFSPLLPVQTIKEEEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTSQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ

PTMs - Q08050 基板として

Site PTM Type Enzyme
S4 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
S35 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
S41 Phosphorylation
K57 Ubiquitination
K63 Acetylation
K189 Ubiquitination
S191 Phosphorylation
K201 Sumoylation
K218 Sumoylation
S251 Phosphorylation P06493 (CDK1)
K278 Methylation
K278 Ubiquitination
K282 Methylation
S331 Phosphorylation
T354 Phosphorylation
K356 Sumoylation
T357 Phosphorylation
S376 Phosphorylation O96017 (CHEK2)
K422 Acetylation
K422 Sumoylation
K422 Ubiquitination
K440 Acetylation
K440 Sumoylation
K440 Ubiquitination
S451 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
K460 Sumoylation
K478 Sumoylation
S481 Phosphorylation
S489 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
K495 Sumoylation
S501 Phosphorylation
S508 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
T510 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
Y517 Phosphorylation
S522 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
T524 Phosphorylation
T600 Phosphorylation P11802 (CDK4) , P06493 (CDK1) , Q00534 (CDK6)
K603 Acetylation
K603 Sumoylation
T611 Phosphorylation P11802 (CDK4) , P06493 (CDK1) , Q00534 (CDK6)
K614 Acetylation
T620 Phosphorylation Q00534 (CDK6) , P11802 (CDK4) , P06493 (CDK1)
S623 Phosphorylation
T627 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
S638 Phosphorylation
S672 Phosphorylation
S692 Phosphorylation
S693 Phosphorylation
S704 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
S717 Phosphorylation
T719 Phosphorylation
S730 Phosphorylation P53350 (PLK1)
S739 Phosphorylation P53350 (PLK1)

研究背景

機能:

Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response.

PTMs:

Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.

タンパク質ファミリー:

Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.

研究領域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

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