製品: PDHK1 Mouse Monoclonal Antibody
カタログ: BF8346
タンパク質の説明: Mouse monoclonal antibody to PDHK1
アプリケーション: WB IHC
反応性: Human, Rat
分子量: 50kD; 49kD(Calculated).
ユニプロット: Q15118

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Monoclonal [AFfirm8346]
特異性:
PDHK1 Mouse Monoclonal Antibody detects endogenous levels of total PDHK1.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDH kinase 1; Pdk1; PDK1_HUMAN; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q15118 PDK1_HUMAN:

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

タンパク質配列:
MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA

PTMs - Q15118 基板として

Site PTM Type Enzyme
S25 Phosphorylation
S67 Phosphorylation
K133 Ubiquitination
Y136 Phosphorylation P11362 (FGFR1)
K230 Ubiquitination
Y243 Phosphorylation P11362 (FGFR1) , O60674 (JAK2) , P00519 (ABL1) , P36888 (FLT3)
Y244 Phosphorylation P11362 (FGFR1)
R319 Methylation
Y336 Phosphorylation
T338 Phosphorylation P00558 (PGK1)
T346 Phosphorylation
S347 Phosphorylation
Y357 Phosphorylation
S393 Phosphorylation
S396 Phosphorylation
Y403 Phosphorylation
K405 Ubiquitination

PTMs - Q15118 酵素として

Substrate Site Source
P05106-1 (ITGB3) T779 Uniprot
P08559 (PDHA1) S232 Uniprot
P08559 (PDHA1) S293 Uniprot
P08559 (PDHA1) S300 Uniprot
P23443-1 (RPS6KB1) T390 Uniprot
P29803 (PDHA2) S230 Uniprot
P29803 (PDHA2) S291 Uniprot
P29803 (PDHA2) S298 Uniprot
P31749-1 (AKT1) T308 Uniprot
P31749-1 (AKT1) S473 Uniprot
P31751 (AKT2) T309 Uniprot
Q15118-1 (PDK1) S25 Uniprot
Q15118-1 (PDK1) S393 Uniprot
Q15118-1 (PDK1) S396 Uniprot
Q16513 (PKN2) T816 Uniprot
Q16513-1 (PKN2) T951 Uniprot
Q9Y243-1 (AKT3) S120 Uniprot
Q9Y243 (AKT3) T305 Uniprot

研究背景

機能:

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

PTMs:

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

サブユニット構造:

Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).

タンパク質ファミリー:

Belongs to the PDK/BCKDK protein kinase family.

研究領域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

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