AFfirm™ PDHK1 Mouse Monoclonal Antibody - #BF8346
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; PDH kinase 1; Pdk1; PDK1_HUMAN; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isoenzyme 1;
免疫原
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
- Q15118 PDK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA
PTMs - Q15118 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S25 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| Y136 | Phosphorylation | P11362 (FGFR1) | Uniprot |
| K230 | Ubiquitination | Uniprot | |
| Y243 | Phosphorylation | P11362 (FGFR1) , O60674 (JAK2) , P00519 (ABL1) , P36888 (FLT3) | Uniprot |
| Y244 | Phosphorylation | P11362 (FGFR1) | Uniprot |
| R319 | Methylation | Uniprot | |
| Y336 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | P00558 (PGK1) | Uniprot |
| T346 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| S393 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| Y403 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot |
PTMs - Q15118 酵素として
| Substrate | Site | Source |
|---|---|---|
| P05106-1 (ITGB3) | T779 | Uniprot |
| P08559 (PDHA1) | S232 | Uniprot |
| P08559 (PDHA1) | S293 | Uniprot |
| P08559 (PDHA1) | S300 | Uniprot |
| P23443-1 (RPS6KB1) | T390 | Uniprot |
| P29803 (PDHA2) | S230 | Uniprot |
| P29803 (PDHA2) | S291 | Uniprot |
| P29803 (PDHA2) | S298 | Uniprot |
| P31749-1 (AKT1) | T308 | Uniprot |
| P31749-1 (AKT1) | S473 | Uniprot |
| P31751 (AKT2) | T309 | Uniprot |
| Q15118-1 (PDK1) | S25 | Uniprot |
| Q15118-1 (PDK1) | S393 | Uniprot |
| Q15118-1 (PDK1) | S396 | Uniprot |
| Q16513 (PKN2) | T816 | Uniprot |
| Q16513-1 (PKN2) | T951 | Uniprot |
| Q9Y243-1 (AKT3) | S120 | Uniprot |
| Q9Y243 (AKT3) | T305 | Uniprot |
研究背景
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect.
Mitochondrion matrix.
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.
Homodimer, and heterodimer with PDK2. Interacts with the pyruvate dehydrogenase complex subunit DLAT, and is part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3).
Belongs to the PDK/BCKDK protein kinase family.
研究領域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.