AFfirm™ SRF Mouse Monoclonal Antibody - #BF8229
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN;
免疫原
- P11831 SRF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE
PTMs - P11831 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T4 | Phosphorylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| R24 | Methylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| R37 | Methylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| Y76 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S79 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S83 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S85 | Phosphorylation | P49137 (MAPKAPK2) , P68400 (CSNK2A1) | Uniprot |
| S101 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Q15418 (RPS6KA1) , O75582 (RPS6KA5) , Q9UQM7 (CAMK2A) , P49137 (MAPKAPK2) | Uniprot |
| K147 | Sumoylation | Uniprot | |
| T159 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) , Q09013 (DMPK) , P17252 (PRKCA) | Uniprot |
| T160 | Phosphorylation | Q9UQM7 (CAMK2A) , Q05655 (PRKCD) | Uniprot |
| S162 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S277 | O-Glycosylation | Uniprot | |
| S307 | O-Glycosylation | Uniprot | |
| S309 | O-Glycosylation | Uniprot | |
| S313 | O-Glycosylation | Uniprot | |
| S316 | O-Glycosylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| S383 | O-Glycosylation | Uniprot | |
| T401 | O-Glycosylation | Uniprot | |
| S435 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S446 | Phosphorylation | P78527 (PRKDC) | Uniprot |
研究背景
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.
Phosphorylated by PRKDC.
Nucleus.
Binds DNA as a multimer, probably a dimer. Interacts with MRTFA, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF. Forms a nuclear ternary complex with MRTFA and SCAI. Interacts with MRTFB. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 (By similarity). Interacts with SRFBP1 (By similarity). Interacts with FOXK1. Interacts with LPXN. Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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