AFfirm™ MCM2 Mouse Monoclonal Antibody - #BF8427
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
BM28; CCNL 1; CCNL1; CDC like 1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050;
免疫原
A synthesized peptide derived from human MCM2.
- P49736 MCM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF
PTMs - P49736 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| S5 | Phosphorylation | P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| S7 | Phosphorylation | P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| T9 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) | Uniprot |
| T25 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) | Uniprot |
| S31 | Phosphorylation | O00311 (CDC7) | Uniprot |
| S32 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) , P24941 (CDK2) | Uniprot |
| S41 | Phosphorylation | O00311 (CDC7) , P24941 (CDK2) , P68400 (CSNK2A1) , P50613 (CDK7) | Uniprot |
| S53 | Phosphorylation | P68400 (CSNK2A1) , P24941 (CDK2) , P50613 (CDK7) , O00311 (CDC7) | Uniprot |
| T59 | Phosphorylation | P50613 (CDK7) | Uniprot |
| Y81 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , Q13535 (ATR) , O00311 (CDC7) | Uniprot |
| R126 | Methylation | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) | Uniprot |
| T158 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S220 | Phosphorylation | O00311 (CDC7) , O14965 (AURKA) | Uniprot |
| K224 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y234 | Phosphorylation | Uniprot | |
| R307 | Methylation | Uniprot | |
| C315 | S-Nitrosylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K469 | Acetylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| K613 | Ubiquitination | Uniprot | |
| R623 | Methylation | Uniprot | |
| R677 | Methylation | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| K742 | Acetylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| K745 | Ubiquitination | Uniprot | |
| R751 | Methylation | Uniprot | |
| K752 | Ubiquitination | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| T757 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| S769 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| T857 | Phosphorylation | Uniprot | |
| K863 | Ubiquitination | Uniprot | |
| K868 | Ubiquitination | Uniprot | |
| S878 | Phosphorylation | Uniprot | |
| K896 | Acetylation | Uniprot | |
| K896 | Ubiquitination | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Nucleus.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.
Belongs to the MCM family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
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