TAF4 Antibody - #AF0305
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0305, RRID:AB_2833469.
折りたたみ／展開
TAF2C; TAF2C1; TAF4A; TAFII 130; TAFII 135; TBP associated factor 4; TAF(II)105; TAF2C2; TAF4b RNA polymerase II, TATA box binding protein (TBP) associated factor, 105kDa; TAFII 105; TAFII105; TAFII105ALPHA; TATA box binding protein (TBP) associated factor 4B; TATA box binding protein (TBP) associated factor, RNA polymerase II, C2, 105kD; Transcription initiation factor TFIID 105 kD subunit; Transcription initiation factor TFIID 105 kDa subunit; Transcription initiation factor TFIID subunit 4B;
免疫原
Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.
- O00268 TAF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAGSDLLDEVFFNSEVDEKVVSDLVGSLESQLAASAAHHHHLAPRTPEVRAAAAGALGNHVVSGSPAGAAGAGPAAPAEGAPGAAPEPPPAGRARPGGGGPQRPGPPSPRRPLVPAGPAPPAAKLRPPPEGSAGSCAPVPAAAAVAAGPEPAPAGPAKPAGPAALAARAGPGPGPGPGPGPGPGPGKPAGPGAAQTLNGSAALLNSHHAAAPAVSLVNNGPAALLPLPKPAAPGTVIQTPPFVGAAAPPAPAAPSPPAAPAPAAPAAAPPPPPPAPATLARPPGHPAGPPTAAPAVPPPAAAQNGGSAGAAPAPAPAAGGPAGVSGQPGPGAAAAAPAPGVKAESPKRVVQAAPPAAQTLAASGPASTAASMVIGPTMQGALPSPAAVPPPAPGTPTGLPKGAAGAVTQSLSRTPTATTSGIRATLTPTVLAPRLPQPPQNPTNIQNFQLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCKNFLSTLIKLASSGKQSTETAANVKELVQNLLDGKIEAEDFTSRLYRELNSSPQPYLVPFLKRSLPALRQLTPDSAAFIQQSQQQPPPPTSQATTALTAVVLSSSVQRTAGKTAATVTSALQPPVLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQPHNRIMLTTPQQIQLNPLQPVPVVKPAVLPGTKALSAVSAQAAAAQKNKLKEPGGGSFRDDDDINDVASMAGVNLSEESARILATNSELVGTLTRSCKDETFLLQAPLQRRILEIGKKHGITELHPDVVSYVSHATQQRLQNLVEKISETAQQKNFSYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDEQEREILMRAAKSRSRQEDPEQLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRKKRKVDCPGPGSGAEGSGPGSVVPGSSGVGTPRQFTRQRITRVNLRDLIFCLENERETSHSLLLYKAFLK
- Q92750 TAF4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPAGLTEPAGAAPPAAVSASGTVTMAPAGALPVRVESTPVALGAVTKAPVSVCVEPTASQPLRSPVGTLVTKVAPVSAPPKVSSGPRLPAPQIVAVKAPNTTTIQFPANLQLPPGTVLIKSNSGPLMLVSPQQTVTRAETTSNITSRPAVPANPQTVKICTVPNSSSQLIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLENVKKCKNFLAMLIKLACSGSQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQTSSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAATGGTTAGTGLLQTSKPLVTSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVKQLVVQQPSGGNEKQVTTISHSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDINDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLTAIAQHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRKKRPLESGIEGLKDNLLASGTSSLTATKQLHRPRITRICLRDLIFCMEQEREMKYSRALYLALLK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00268/Q92750 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S23 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| R414 | Methylation | Uniprot | |
| T415 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| R424 | Methylation | Uniprot | |
| R435 | Methylation | Uniprot | |
| R491 | Methylation | Uniprot | |
| R516 | Methylation | Uniprot | |
| S528 | O-Glycosylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| T571 | Phosphorylation | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| Y652 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| R754 | Methylation | Uniprot | |
| R769 | Methylation | Uniprot | |
| K795 | Acetylation | Uniprot | |
| S827 | Phosphorylation | Uniprot | |
| S857 | Phosphorylation | Uniprot | |
| T862 | Phosphorylation | Uniprot | |
| K945 | Ubiquitination | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| S1032 | Phosphorylation | Uniprot | |
| S1042 | Phosphorylation | Uniprot | |
| T1046 | Phosphorylation | Uniprot | |
| T1056 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R63 | Methylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| R87 | Methylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| T395 | Phosphorylation | Uniprot | |
| S489 | O-Glycosylation | Uniprot | |
| T502 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K670 | Ubiquitination | Uniprot | |
| K731 | Ubiquitination | Uniprot | |
| K799 | Ubiquitination | Uniprot |
研究背景
Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
Nucleus.
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with ATF7; the interaction inhibits ATF7-mediated tranactivation.
(Microbial infection) Interacts with SV40 Large T antigen.
Belongs to the TAF4 family.
Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF-kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis.
Under stimulation by forskolin, Isoform 1 is phosphorylated by protein kinase A (PKA).
Nucleus. Cytoplasm.
Note: Export into the cytoplasm is mediated by a CRM1-independent nuclear export pathway and not by phosphorylation.
Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells.
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain. This heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer. Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers. Interaction with POU2AF1, via its C-terminal activation domain, is required for octamer-dependent transcription.
Belongs to the TAF4 family.
研究領域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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