MAP2 Antibody - #AF4081

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製品: MAP2 Antibody
カタログ: AF4081
タンパク質の説明: Rabbit polyclonal antibody to MAP2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 55-75kD(2c/2d), 280kD(2a/2b); 200kD(Calculated).
ユニプロット: P11137
RRID: AB_2835352

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 200ul $450 在庫あり

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:1000, IF/ICC 1:200, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
MAP2 Antibody detects endogenous levels of total MAP2.
RRID:
AB_2835352
引用形式: Affinity Biosciences Cat# AF4081, RRID:AB_2835352.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp686I2148; MAP 2; MAP dendrite specific; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; MTAP2_HUMAN;

免疫原

免疫原:
Uniprot:

P11137(MTAP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
MAP2(4133)
タンパク質の説明:
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
タンパク質配列:
MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTYPDKKDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKDEWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFLQPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEAINQETVQQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSEQKDQEPTTDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGAATSAELDMPFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQTGKESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLGGRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCFPVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSETVVEDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFYEGTDDKVRRDLATDLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEKSEEHADSKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVEQGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTKVSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEIPCPPAVSEADLATDERADVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQGEYDKLLFRSDTLQITDLGVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRFAALEQPEVERRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDDSIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKEPSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
71
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11137 基板として

Site PTM Type Enzyme
Y50 Phosphorylation
S63 Phosphorylation
T66 Phosphorylation
Y67 Phosphorylation P07948 (LYN) , P06241 (FYN)
S136 Phosphorylation
T168 Phosphorylation
T216 Phosphorylation
S233 Phosphorylation
T241 Phosphorylation
T258 Phosphorylation
S285 Phosphorylation
T290 Phosphorylation
S311 Phosphorylation
S315 Phosphorylation
S321 Phosphorylation
T323 Phosphorylation
S357 Phosphorylation
T361 Phosphorylation
S385 Phosphorylation Q9P0L2 (MARK1)
T427 Phosphorylation
T516 Phosphorylation
S532 Phosphorylation
S585 Phosphorylation
Y592 Phosphorylation
Y593 Phosphorylation
S610 Phosphorylation
S629 Phosphorylation
S725 Phosphorylation
S785 Phosphorylation
S788 Phosphorylation
S821 Phosphorylation
S823 Phosphorylation
S833 Phosphorylation
S843 Phosphorylation
T860 Phosphorylation
S862 Phosphorylation
S881 Phosphorylation
S913 Phosphorylation
Y996 Phosphorylation
S1005 Phosphorylation
S1021 Phosphorylation
S1031 Phosphorylation
S1106 Phosphorylation
T1108 Phosphorylation
S1139 Phosphorylation
T1154 Phosphorylation
S1155 Phosphorylation
S1158 Phosphorylation
S1234 Phosphorylation
S1324 Phosphorylation
S1347 Phosphorylation
S1353 Phosphorylation
T1366 Phosphorylation
T1368 Phosphorylation
Y1369 Phosphorylation
S1398 Phosphorylation
S1420 Phosphorylation
S1421 Phosphorylation
K1427 Acetylation
T1440 Phosphorylation
K1447 Ubiquitination
K1448 Ubiquitination
S1480 Phosphorylation
Y1493 Phosphorylation
T1494 Phosphorylation
T1497 Phosphorylation
S1500 Phosphorylation
K1505 Acetylation
T1506 Phosphorylation
K1524 Acetylation
S1534 Phosphorylation
S1539 Phosphorylation
S1540 Phosphorylation
S1546 Phosphorylation
S1555 Phosphorylation
S1562 Phosphorylation
S1564 Phosphorylation
S1588 Phosphorylation
T1590 Phosphorylation
S1591 Phosphorylation
T1592 Phosphorylation
T1594 Phosphorylation
T1595 Phosphorylation
S1598 Phosphorylation
T1599 Phosphorylation
T1602 Phosphorylation
T1605 Phosphorylation
S1608 Phosphorylation
Y1609 Phosphorylation
S1611 Phosphorylation
T1613 Phosphorylation
T1616 Phosphorylation
T1619 Phosphorylation
S1621 Phosphorylation
Y1622 Phosphorylation
T1628 Phosphorylation
T1631 Phosphorylation
S1634 Phosphorylation
S1640 Phosphorylation
T1649 Phosphorylation
S1653 Phosphorylation
T1656 Phosphorylation
S1675 Phosphorylation
S1679 Phosphorylation P17612 (PRKACA) , Q9P0L2 (MARK1)
T1680 Phosphorylation
S1702 Phosphorylation
S1706 Phosphorylation
S1710 Phosphorylation Q9P0L2 (MARK1) , P17612 (PRKACA)
S1727 Phosphorylation
S1742 Phosphorylation P17612 (PRKACA)
T1780 Phosphorylation
S1782 Phosphorylation P17612 (PRKACA)
S1786 Phosphorylation
S1787 Phosphorylation
S1790 Phosphorylation
S1795 Phosphorylation
S1798 Phosphorylation
S1799 Phosphorylation
S1800 Phosphorylation
S1802 Phosphorylation
S1808 Phosphorylation
T1813 Phosphorylation
T1819 Phosphorylation

研究背景

機能:

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

PTMs:

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites.

参考文献

1). An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury. Bioactive Materials, 2022 (PubMed: 35845318) [IF=18.9]
2). MicroRNA-124 negatively regulates STAT3 to alleviate hypoxic-ischemic brain damage by inhibiting oxidative stress. Aging (Albany NY), 2024 (PubMed: 38319722) [IF=5.2]

Application: IF/ICC    Species: Rat    Sample:

Figure 1 Expression of miR-124 and cell viability in OGD/R-induced neurons. (A) The change of primary neurons cultured up to day 1, day 3, day 5, and day 7; (B) Representative immunofluorescence images of Map-2 (green) and DAPI (blue) co-staining the primary cortical neurons. Scale bar, 50μm; (C) The Cell viability of the primary cortical neurons under OGD treatment for 0, 1, 2, and 3 h were detected by CCK-8 assay; (D) qRT-PCR analysis of miR-124 levels the primary cortical neurons under OGD treatment for 0, 1, 2, and 3 h; (E) the change between normal neurons and neurons after OGD(2h)/R treatment. Data were expressed as mean ± SD (derived from three independent experiments for each sample). NS, not significant for p > 0.05, * p < 0.05, ** p < 0.01, and *** p < 0.001 (one-way analysis of variance with Tukey’s post hoc tests).
3). Kapβ2 Inhibits Perioperative Neurocognitive Disorders in Rats with Mild Cognitive Impairment by Reversing the Nuclear-Cytoplasmic Mislocalization of hnRNPA2/B1. Molecular neurobiology, 2023 (PubMed: 38102516) [IF=5.1]
4). Fucosyltransferase 9 promotes neuronal differentiation and functional recovery after spinal cord injury by suppressing the activation of Notch signaling: Fucosyltransferase 9 promotes neuronal differentiation. Acta Biochimica et Biophysica Sinica, 2023 (PubMed: 37674364) [IF=3.7]

Application: IF/ICC    Species: Human    Sample:

Figure 3 Overexpression of Fut9 promotes neuronal differentiation (A,B) mRNA and protein expression levels of Fut9 in the NT, LV-Con, and LV-Fut9 groups. (C) Immunofluorescence staining for neural-differentiated markers of NSCs in the NT, LV-Con, and LV-Fut9 groups. Scale bar: 100 μm. (D,E) Quantification of dendritic length and neural-differentiated marker-positive cells of NSCs. (F,G) mRNA and protein expression levels of NF200, β3-tubulin and MAP2 in the NT, LV-Con, and LV-Fut9 groups. (H) Quantification of neural-differentiated marker expression by western blot analysis. Data are presented as the mean±SD from one representative experiment of three independent experiments performed in triplicate. *P
5). Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation. JOURNAL OF MOLECULAR NEUROSCIENCE, 2018 (PubMed: 29243061) [IF=3.1]
6). Targeting neuronal nitric oxide synthase by a cell penetrating peptide Tat-LK15/siRNA bioconjugate. Neuroscience Letters, 2017 (PubMed: 28450191) [IF=2.5]

Application: IF/ICC    Species: rat    Sample:

Fig. 4. Effect of Tat-Lk15 siRNA in RNdsc cells. FAM-modified siRNA was conjugated with Tat-LK15, and then the complex was transfected to RGC5 cells. After transfection for 24 h, the fluorescence intensity of the complex was determined by immunofluorescence microscopy. MAP2 was used to mark RNdsc cells and DAPI staining was used to mark cell nucleus. A–D: ×100, E–L: ×200. I–L was transfected by naked FAM-siRNA.
7). Neuroprotective effect of soyasaponin Bb pretreatment in cerebral ischemia / reperfusion model rats. 中国比较医学杂志, 2023

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