AFfirm™ UMP/CMP kinase Mouse Monoclonal Antibody - #BF8457
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
CMK; CMPK 1; CMPK; cmpk1; Cytidine monophosphate (UMP CMP) kinase 1 cytosolic; Cytidine monophosphate kinase; Cytidine monophosphate UMP CMP kinase 1 cytosolic; Cytidylate kinase; dCMP kinase; Deoxycytidylate kinase; EC 2.7.4.14; KCY; KCY_HUMAN; Monophosphatase kinase; OTTHUMP00000009565; RP11 511I2.1; UCK; UMK; UMP CMP; UMP CMP kinase; UMP-CMP kinase; UMP/CMP kinase; UMP/CMPK; UMPK; Uridine monophosphate kinase; Uridine monophosphate/cytidine monophosphate kinase;
免疫原
- P30085 KCY_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG
PTMs - P30085 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K26 | Ubiquitination | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K43 | Ubiquitination | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y56 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K106 | Methylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| Y166 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot |
研究背景
Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Nucleus. Cytoplasm.
Note: Predominantly cytoplasmic, less than 15% nuclear.
Ubiquitously expressed.
Monomer.
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Belongs to the adenylate kinase family. UMP-CMP kinase subfamily.
研究領域
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.
· Metabolism > Global and overview maps > Metabolic pathways.
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