AFfirm™ CAMKK1 Mouse Monoclonal Antibody - #BF8465
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Ca2+/calmodulin-dependent protein kinase kinase alpha; Calcium/calmodulin dependent protein kinase kinase; Calcium/calmodulin dependent protein kinase kinase 1 alpha; Calcium/calmodulin dependent protein kinase kinase 1; Calcium/calmodulin dependent protein kinase kinase alpha; Calcium/calmodulin-dependent protein kinase kinase 1; Calcium/calmodulin-dependent protein kinase kinase alpha; CaM kinase kinase alpha; CaM-kinase IV kinase; CaM-kinase kinase 1; CaM-kinase kinase alpha; CaM-KK 1; CaM-KK alpha; CaMKK 1; CaMKK alpha; CAMKK alpha protein; CaMKK; Camkk1; CAMKKA; DKFZp761M0423; KKCC1_HUMAN; MGC34095; OTTMUSP00000006419;
免疫原
- Q8N5S9 KKCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSARKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSEEEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLVKEGFGEGGKSPELPGVQEDEAAS
PTMs - Q8N5S9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S52 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S82 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| R102 | Methylation | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| R469 | Methylation | Uniprot | |
| R472 | Methylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S492 | Phosphorylation | Uniprot |
PTMs - Q8N5S9 酵素として
研究背景
Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.
Appears to be autophosphorylated in a Ca(2+)/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca(2+)/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4 (By similarity).
Cytoplasm. Nucleus.
Interacts with CAMK4 and calmodulin.
The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
研究領域
· Human Diseases > Substance dependence > Alcoholism.
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