AFfirm™ BAP1 Mouse Monoclonal Antibody - #BF8460
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
BAP 1; Bap1; BAP1_HUMAN; BRCA 1 associated protein 1; BRCA1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 13; Cerebral protein 6; DKFZp686N04275; FLJ35406; FLJ37180; HUCEP 13; Hucep 6; HUCEP13; Hucep6; KIAA0272; TPDS; Ubiquitin carboxy terminal hydrolase; Ubiquitin carboxyl terminal hydrolase BAP 1; Ubiquitin carboxyl terminal hydrolase BAP1; Ubiquitin carboxyl-terminal hydrolase BAP1; UCHL2;
PTMs - Q92560 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K120 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K155 | Acetylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| T273 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| Y401 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| S485 | Phosphorylation | Uniprot | |
| T487 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| T517 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| T524 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| S592 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| K656 | Methylation | Uniprot | |
| K658 | Methylation | Uniprot | |
| K659 | Methylation | Uniprot | |
| S705 | Phosphorylation | Uniprot |
研究背景
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration. Acts as a tumor suppressor.
Ubiquitinated: monoubiquitinated at multiple site of its nuclear localization signal (NLS) BY UBE2O, leading to cytoplasmic retention. Able to mediate autodeubiquitination via intramolecular interactions to couteract cytoplasmic retention.
Cytoplasm. Nucleus.
Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).
Highly expressed in testis, placenta and ovary. Expressed in breast.
Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1. Interacts (when phosphorylated at Thr-493) with FOXK1. Interacts (when phosphorylated at Thr-493) with FOXK2; leading to recruit the PR-DUB complex and repress FOXK2 target genes.
Belongs to the peptidase C12 family. BAP1 subfamily.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.