AFfirm™ ORC3 Mouse Monoclonal Antibody - #BF8479
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Homolog of Latheo; homolog of latheo, Drosophila; LAT; LATHEO; LATHEO, drosophila, homolog of; orc3; ORC3_HUMAN; ORC3L; Origin recognition complex subunit 3; Origin recognition complex subunit 3 honolog; Origin Recognition Complex Subunit 3 Isoform 1; Origin recognition complex subunit 3 like; Origin recognition complex subunit Latheo; Origin Recognition Complex, subunit 3, S. cerevisiae, homolog of; Origin recognition complex, subunit 3-like (S. cerevisiae); Origin recognition complex, subunit 3-like (yeast);
免疫原
- Q9UBD5 ORC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATSSMSKGCFVFKPNSKKRKISLPIEDYFNKGKNEPEDSKLRFETYQLIWQQMKSENERLQEELNKNLFDNLIEFLQKSHSGFQKNSRDLGGQIKLREIPTAALVLGVNVTDHDLTFGSLTEALQNNVTPYVVSLQAKDCPDMKHFLQKLISQLMDCCVDIKSKEEESVHVTQRKTHYSMDSLSSWYMTVTQKTDPKMLSKKRTTSSQWQSPPVVVILKDMESFATKVLQDFIIISSQHLHEFPLILIFGIATSPIIIHRLLPHAVSSLLCIELFQSLSCKEHLTTVLDKLLLTTQFPFKINEKVLQVLTNIFLYHDFSVQNFIKGLQLSLLEHFYSQPLSVLCCNLPEAKRRINFLSNNQCENIRRLPSFRRYVEKQASEKQVALLTNERYLKEETQLLLENLHVYHMNYFLVLRCLHKFTSSLPKYPLGRQIRELYCTCLEKNIWDSEEYASVLQLLRMLAKDELMTILEKCFKVFKSYCENHLGSTAKRIEEFLAQFQSLDETKEEEDASGSQPKGLQKTDLYHLQKSLLEMKELRRSKKQTKFEVLRENVVNFIDCLVREYLLPPETQPLHEVVYFSAAHALREHLNAAPRIALHTALNNPYYYLKNEALKSEEGCIPNIAPDICIAYKLHLECSRLINLVDWSEAFATVVTAAEKMDANSATSEEMNEIIHARFIRAVSELELLGFIKPTKQKTDHVARLTWGGC
PTMs - Q9UBD5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K21 | Acetylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y29 | Phosphorylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| Y47 | Phosphorylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K145 | Acetylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| K543 | Acetylation | Uniprot | |
| Y607 | Phosphorylation | Uniprot | |
| K611 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| K694 | Ubiquitination | Uniprot |
研究背景
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3.
Multi-mono-ubiquitinated by OBI1; ubiquitination is important for efficient DNA replication origin site activation. Ubiquitination levels are low in mitotic and early G1-phAse cells and are induced in late G1-/early S-phase, peaking in S-phase and decrease toward the end of the cell cycle.
Nucleus. Chromosome.
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.
Belongs to the ORC3 family.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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