AFfirm™ PIKFYVE Mouse Monoclonal Antibody - #BF8480

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製品: PIKFYVE Mouse Monoclonal Antibody
カタログ: BF8480
タンパク質の説明: Mouse monoclonal antibody to PIKFYVE
アプリケーション: WB IHC IF/ICC
反応性: Human, Rat
分子量: 250kD; 237kD(Calculated).
ユニプロット: Q9Y2I7

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Monoclonal [AFfirm8480]
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

1 phosphatidylinositol 3 phosphate 5 kinase; 1 phosphatidylinositol 4 phosphate 5 kinase; 1-phosphatidylinositol 3-phosphate 5-kinase; CFD; epididymis luminal protein 37; FAB1; FAB1, S. cerevisiae, homolog of; FYV1_HUMAN; FYVE finger containing phosphoinositide kinase; FYVE finger-containing phosphoinositide kinase; KIAA0981; MGC40423; p235; Phosphatidylinositol 3 phosphate 5 kinase type III; Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III; Phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase type III; Phosphatidylinositol 4 phosphate 5 kinase type III; Phosphoinositide kinase, FYVE finger containing; PIKfyve; PIP5K; PIP5K3; PIPkin III; PIPkin-III; PtdIns(4)P 5 kinase; Type III PIP kinase; ZFYVE29; Zinc finger, FYVE domain containing 29;

免疫原

免疫原:

A synthesized peptide derived from Human PIKFYVE.

Uniprot:

Q9Y2I7(FYV1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PIKFYVE(200576)
タンパク質配列:
MATDDKTSPTLDSANDLPRSPTSPSHLTHFKPLTPDQDEPPFKSAYSSFVNLFRFNKERAEGGQGEQQPLSGSWTSPQLPSRTQSVRSPTPYKKQLNEELQRRSSALDTRRKAEPTFGGHDPRTAVQLRSLSTVLKRLKEIMEGKSQDSDLKQYWMPDSQCKECYDCSEKFTTFRRRHHCRLCGQIFCSRCCNQEIPGKFMGYTGDLRACTYCRKIALSYAHSTDSNSIGEDLNALSDSACSVSVLDPSEPRTPVGSRKASRNIFLEDDLAWQSLIHPDSSNTPLSTRLVSVQEDAGKSPARNRSASITNLSLDRSGSPMVPSYETSVSPQANRTYVRTETTEDERKILLDSVQLKDLWKKICHHSSGMEFQDHRYWLRTHPNCIVGKELVNWLIRNGHIATRAQAIAIGQAMVDGRWLDCVSHHDQLFRDEYALYRPLQSTEFSETPSPDSDSVNSVEGHSEPSWFKDIKFDDSDTEQIAEEGDDNLANSASPSKRTSVSSFQSTVDSDSAASISLNVELDNVNFHIKKPSKYPHVPPHPADQKEYLISDTGGQQLSISDAFIKESLFNRRVEEKSKELPFTPLGWHHNNLELLREENGEKQAMERLLSANHNHMMALLQQLLHSDSLSSSWRDIIVSLVCQVVQTVRPDVKNQDDDMDIRQFVHIKKIPGGKKFDSVVVNGFVCTKNIAHKKMSSCIKNPKILLLKCSIEYLYREETKFTCIDPIVLQEREFLKNYVQRIVDVRPTLVLVEKTVSRIAQDMLLEHGITLVINVKSQVLERISRMTQGDLVMSMDQLLTKPHLGTCHKFYMQIFQLPNEQTKTLMFFEGCPQHLGCTIKLRGGSDYELARVKEILIFMICVAYHSQLEISFLMDEFAMPPTLMQNPSFHSLIEGRGHEGAVQEQYGGGSIPWDPDIPPESLPCDDSSLLELRIVFEKGEQENKNLPQAVASVKHQEHSTTACPAGLPCAFFAPVPESLLPLPVDDQQDALGSEQPETLQQTVVLQDPKSQIRAFRDPLQDDTGLYVTEEVTSSEDKRKTYSLAFKQELKDVILCISPVITFREPFLLTEKGMRCSTRDYFAEQVYWSPLLNKEFKEMENRRKKQLLRDLSGLQGMNGSIQAKSIQVLPSHELVSTRIAEHLGDSQSLGRMLADYRARGGRIQPKNSDPFAHSKDASSTSSGQSGSKNEGDEERGLILSDAVWSTKVDCLNPINHQRLCVLFSSSSAQSSNAPSACVSPWIVTMEFYGKNDLTLGIFLERYCFRPSYQCPSMFCDTPMVHHIRRFVHGQGCVQIILKELDSPVPGYQHTILTYSWCRICKQVTPVVALSNESWSMSFAKYLELRFYGHQYTRRANAEPCGHSIHHDYHQYFSYNQMVASFSYSPIRLLEVCVPLPKIFIKRQAPLKVSLLQDLKDFFQKVSQVYVAIDERLASLKTDTFSKTREEKMEDIFAQKEMEEGEFKNWIEKMQARLMSSSVDTPQQLQSVFESLIAKKQSLCEVLQAWNNRLQDLFQQEKGRKRPSVPPSPGRLRQGEESKISAMDASPRNISPGLQNGEKEDRFLTTLSSQSSTSSTHLQLPTPPEVMSEQSVGGPPELDTASSSEDVFDGHLLGSTDSQVKEKSTMKAIFANLLPGNSYNPIPFPFDPDKHYLMYEHERVPIAVCEKEPSSIIAFALSCKEYRNALEELSKATQWNSAEEGLPTNSTSDSRPKSSSPIRLPEMSGGQTNRTTETEPQPTKKASGMLSFFRGTAGKSPDLSSQKRETLRGADSAYYQVGQTGKEGTENQGVEPQDEVDGGDTQKKQLINPHVELQFSDANAKFYCRLYYAGEFHKMREVILDSSEEDFIRSLSHSSPWQARGGKSGAAFYATEDDRFILKQMPRLEVQSFLDFAPHYFNYITNAVQQKRPTALAKILGVYRIGYKNSQNNTEKKLDLLVMENLFYGRKMAQVFDLKGSLRNRNVKTDTGKESCDVVLLDENLLKMVRDNPLYIRSHSKAVLRTSIHSDSHFLSSHLIIDYSLLVGRDDTSNELVVGIIDYIRTFTWDKKLEMVVKSTGILGGQGKMPTVVSPELYRTRFCEAMDKYFLMVPDHWTGLGLNC

PTMs - Q9Y2I7 基板として

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
T7 Phosphorylation
S20 Phosphorylation
T22 Phosphorylation
S23 Phosphorylation
T34 Phosphorylation
K43 Methylation
S44 Phosphorylation
Y46 Phosphorylation
S48 Phosphorylation
S76 Phosphorylation
T83 Phosphorylation
S88 Phosphorylation
T90 Phosphorylation
Y92 Phosphorylation
K94 Ubiquitination
S104 Phosphorylation
S105 Phosphorylation
T109 Phosphorylation
K112 Ubiquitination
S132 Phosphorylation
T133 Phosphorylation
K136 Ubiquitination
K139 Ubiquitination
K152 Ubiquitination
Y154 Phosphorylation
T173 Phosphorylation
T253 Phosphorylation
S261 Phosphorylation
S274 Phosphorylation
S291 Phosphorylation
K298 Ubiquitination
S299 Phosphorylation
S305 Phosphorylation
S307 Phosphorylation P54646 (PRKAA2) , P31749 (AKT1)
T309 Phosphorylation
S312 Phosphorylation
S316 Phosphorylation
S318 Phosphorylation P31749 (AKT1) , O00141 (SGK1)
Y324 Phosphorylation
T326 Phosphorylation
S327 Phosphorylation
S329 Phosphorylation
T335 Phosphorylation
T339 Phosphorylation
T341 Phosphorylation
T342 Phosphorylation
K347 Ubiquitination
S352 Phosphorylation
K356 Ubiquitination
K361 Ubiquitination
S366 Phosphorylation
S367 Phosphorylation
S449 Phosphorylation
S452 Phosphorylation
S454 Phosphorylation
S457 Phosphorylation
S475 Phosphorylation
T477 Phosphorylation
S491 Phosphorylation
S493 Phosphorylation
K565 Ubiquitination
K578 Ubiquitination
T583 Phosphorylation
K669 Acetylation
K675 Acetylation
K720 Ubiquitination
K736 Ubiquitination
K754 Ubiquitination
T822 Phosphorylation
S845 Phosphorylation
S888 Phosphorylation
K938 Ubiquitination
K944 Ubiquitination
K1046 Ubiquitination
S1111 Phosphorylation
S1124 Phosphorylation
Y1155 Phosphorylation
K1165 Ubiquitination
S1167 Phosphorylation
S1177 Phosphorylation
K1206 Ubiquitination
S1301 Phosphorylation
T1312 Phosphorylation
Y1350 Phosphorylation
K1414 Ubiquitination
K1435 Ubiquitination
T1436 Phosphorylation
S1440 Phosphorylation
K1441 Ubiquitination
K1446 Ubiquitination
S1474 Phosphorylation
S1475 Phosphorylation
T1479 Phosphorylation
S1489 Phosphorylation
S1496 Phosphorylation
K1516 Ubiquitination
S1522 Phosphorylation
S1526 Phosphorylation
S1544 Phosphorylation
S1549 Phosphorylation
K1689 Ubiquitination
S1712 Phosphorylation
S1714 Phosphorylation
S1741 Phosphorylation
S1754 Phosphorylation
K1761 Ubiquitination
T1764 Phosphorylation
S1770 Phosphorylation
Y1772 Phosphorylation
Y1773 Phosphorylation
K1832 Ubiquitination
K1861 Ubiquitination
K1953 Ubiquitination
K1967 Ubiquitination
T2026 Phosphorylation
Y2037 Phosphorylation
K2062 Ubiquitination
S2068 Phosphorylation

PTMs - Q9Y2I7 酵素として

Substrate Site Source
P31749 (AKT1) S473 Uniprot

研究背景

機能:

The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.

PTMs:

Phosphorylated in response to insulin at Ser-318 in a protein kinase B (PKB)-dependent manner.

細胞の位置付け:

Endosome membrane.
Note: Mainly associated with membranes of the late endocytic pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14. VAC14 nucleates the assembly of the complex and serves as a scaffold.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

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