b-Enolase Antibody - #AF4099
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4099, RRID:AB_2835357.
折りたたみ／展開
2 phospho D glycerate hydrol yase; 2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Beta enolase; Beta-enolase; ENO3; ENOB_HUMAN; Enolase 3 (beta muscle); Enolase 3; Enolase3; GSD13; MSE; Muscle specific enolase; Muscle-specific enolase; Skeletal muscle enolase;
免疫原
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
- P13929 ENOB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P13929 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| T41 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| K60 | Acetylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| Y189 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| Y257 | Phosphorylation | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| Y287 | Phosphorylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| C357 | S-Nitrosylation | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K406 | Ubiquitination | Uniprot | |
| K420 | Ubiquitination | Uniprot |
研究背景
Appears to have a function in striated muscle development and regeneration.
Cytoplasm.
Note: Localized to the Z line. Some colocalization with CKM at M-band (By similarity).
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Interacts with PNKD.
Belongs to the enolase family.
研究領域
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
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