AFfirm™ XRCC4 Mouse Monoclonal Antibody - #BF8549
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
DNA double strand break repair and V(D)J recombination protein XRCC4; DNA repair protein XRCC4; SSMED; X ray repair complementing defective repair in Chinese hamster cells 4; X ray repair cross complementing 4; X ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; XRCC 4; XRCC4; XRCC4_HUMAN;
免疫原
- Q13426 XRCC4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
PTMs - Q13426 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S193 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K210 | Sumoylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| Y229 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| T233 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S237 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T264 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S315 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| T323 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| S328 | Phosphorylation | P78527 (PRKDC) | Uniprot |
研究背景
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
Nucleus.
Widely expressed.
Homodimer and homotetramer in solution. The homodimer associates with LIG4. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts directly with PRKDC but not with the XRCC6/Ku70 and XRCC5/Ku86 dimer. Interacts with APTX and APLF.
Belongs to the XRCC4 family.
研究領域
· Genetic Information Processing > Replication and repair > Non-homologous end-joining.
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