AFfirm™ MMP14 Mouse Monoclonal Antibody - #BF8599
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Matrix metallopeptidase 14 (membrane inserted); Matrix metalloproteinase 14; Matrix metalloproteinase-14; Membrane type 1 matrix metalloproteinase; Membrane type 1 metalloprotease; Membrane type matrix metalloproteinase 1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP 14; MMP X1; MMP-14; MMP-X1; Mmp14; MMP14_HUMAN; MMPX1; MT MMP 1; MT-MMP 1; MT1 MMP; MT1-MMP; MT1MMP; MTMMP 1; MTMMP1;
免疫原
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
- P50281 MMP14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSPAPRPPRCLLLPLLTLGTALASLGSAQSSSFSPEAWLQQYGYLPPGDLRTHTQRSPQSLSAAIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLAHAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQWMDTENFVLPDDDRRGIQQLYGGESGFPTKMPPQPRTTSRPSVPDKPKNPTYGPNICDGNFDTVAMLRGEMFVFKERWFWRVRNNQVMDGYPMPIGQFWRGLPASINTAYERKDGKFVFFKGDKHWVFDEASLEPGYPKHIKELGRGLPTDKIDAALFWMPNGKTYFFRGNKYYRFNEELRAVDSEYPKNIKVWEGIPESPRGSFMGSDEVFTYFYKGNKYWKFNNQKLKVEPGYPKSALRDWMGCPSGGRPDEGTEEETEVIIIEVDEEGGGAVSAAAVVLPVLLLLLVLAVGLAVFFFRRHGTPRRLLYCQRSLLDKV
PTMs - P50281 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T77 | O-Glycosylation | Uniprot | |
| T77 | Phosphorylation | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| T291 | O-Glycosylation | Uniprot | |
| T299 | O-Glycosylation | Uniprot | |
| T300 | O-Glycosylation | Uniprot | |
| S301 | O-Glycosylation | Uniprot | |
| S304 | O-Glycosylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| T567 | Phosphorylation | P05129 (PRKCG) | Uniprot |
| Y573 | Phosphorylation | P12931 (SRC) , P53667 (LIMK1) | Uniprot |
| S577 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot |
研究背景
Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (By similarity). May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.
The precursor is cleaved by a furin endopeptidase.
Tyrosine phosphorylated by PKDCC/VLK.
Membrane>Single-pass type I membrane protein. Melanosome. Cytoplasm.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Interacts (via C-terminal cytoplasmic tail) with BST2. Interacts with DLL1; inhibits DLL1-induced Notch signaling.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
研究領域
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
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