AFfirm™ MAP2 Mouse Monoclonal Antibody - #BF8607
製品説明
ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human
クローナリティ:
Monoclonal [AFfirm8607]
特異性:
MAP2 Mouse Monoclonal Antibody detects endogenous levels of total MAP2.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
DKFZp686I2148; MAP 2; MAP dendrite specific; MAP-2; MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; MTAP2_HUMAN;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質の説明:
Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).
タンパク質配列:
- P11137 MTAP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADERKDEAKAPHWTSAPLTEASAHSHPPEIKDQGGAGEGLVRSANGFPYREDEEGAFGEHGSQGTYSNTKENGINGELTSADRETAEEVSARIVQVVTAEAVAVLKGEQEKEAQHKDQTAALPLAAEETANLPPSPPPSPASEQTVTVEEDLLTASKMEFHDQQELTPSTAEPSDQKEKESEKQSKPGEDLKHAALVSQPETTKTYPDKKDMQGTEEEKAPLALFGHTLVASLEDMKQKTEPSLVVPGIDLPKEPPTPKEQKDWFIEMPTEAKKDEWGLVAPISPGPLTPMREKDVFDDIPKWEGKQFDSPMPSPFQGGSFTLPLDVMKNEIVTETSPFAPAFLQPDDKKSLQQTSGPATAKDSFKIEEPHEAKPDKMAEAPPSEAMTLPKDAHIPVVEEHVMGKVLEEEKEAINQETVQQRDTFTPSGQEPILTEKETELKLEEKTTISDKEAVPKESKPPKPADEEIGIIQTSTEHTFSEQKDQEPTTDMLKQDSFPVSLEQAVTDSAMTSKTLEKAMTEPSALIEKSSIQELFEMRVDDKDKIEGVGAATSAELDMPFYEDKSGMSKYFETSALKEEATKSIEPGSDYYELSDTRESVHESIDTMSPMHKNGDKEFQTGKESQPSPPAQEAGYSTLAQSYPSDLPEEPSSPQERMFTIDPKVYGEKRDLHSKNKDDLTLSRSLGLGGRSAIEQRSMSINLPMSCLDSIALGFNFGRGHDLSPLASDILTNTSGSMDEGDDYLPATTPALEKAPCFPVESKEEEQIEKVKATGEESTQAEISCESPFLAKDFYKNGTVMAPDLPEMLDLAGTRSRLASVSADAEVARRKSVPSETVVEDSRTGLPPVTDENHVIVKTDSQLEDLGYCVFNKYTVPLPSPVQDSENLSGESGTFYEGTDDKVRRDLATDLSLIEVKLAAAGRVKDEFSVDKEASAHISGDKSGLSKEFDQEKKANDRLDTVLEKSEEHADSKEHAKKTEEAGDEIETFGLGVTYEQALAKDLSIPTDASSEKAEKGLSSVPEIAEVEPSKKVEQGLDFAVQGQLDVKISDFGQMASGLNIDDRRATELKLEATQDMTPSSKAPQEADAFMGVESGHMKEGTKVSETEVKEKVAKPDLVHQEAVDKEESYESSGEHESLTMESLKADEGKKETSPESSLIQDEIAVKLSVEIPCPPAVSEADLATDERADVQMEFIQGPKEESKETPDISITPSDVAEPLHETIVSEPAEIQSEEEEIEAQGEYDKLLFRSDTLQITDLGVSGAREEFVETCPSEHKGVIESVVTIEDDFITVVQTTTDEGESGSHSVRFAALEQPEVERRPSPHDEEEFEVEEAAEAQAEPKDGSPEAPASPEREEVALSEYKTETYDDYKDETTIDDSIMDADSLWVDTQDDDRSIMTEQLETIPKEEKAEKEARRSSLEKHRKEKPFKTGRGRISTPERKVAKKEPSTVSRDEVRRKKAVYKKAELAKKTEVQAHSPSRKFILKPAIKYTRPTHLSCVKRKTTAAGGESALAPSVFKQAKDKVSDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL
PTMs - P11137 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y50 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| Y67 | Phosphorylation | P07948 (LYN) , P06241 (FYN) | Uniprot |
| S136 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| T290 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Q9P0L2 (MARK1) | Uniprot |
| T427 | Phosphorylation | Uniprot | |
| T516 | Phosphorylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| Y592 | Phosphorylation | Uniprot | |
| Y593 | Phosphorylation | Uniprot | |
| S610 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| S785 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S821 | Phosphorylation | Uniprot | |
| S823 | Phosphorylation | Uniprot | |
| S833 | Phosphorylation | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| T860 | Phosphorylation | Uniprot | |
| S862 | Phosphorylation | Uniprot | |
| S881 | Phosphorylation | Uniprot | |
| S913 | Phosphorylation | Uniprot | |
| Y996 | Phosphorylation | Uniprot | |
| S1005 | Phosphorylation | Uniprot | |
| S1021 | Phosphorylation | Uniprot | |
| S1031 | Phosphorylation | Uniprot | |
| S1106 | Phosphorylation | Uniprot | |
| T1108 | Phosphorylation | Uniprot | |
| S1139 | Phosphorylation | Uniprot | |
| T1154 | Phosphorylation | Uniprot | |
| S1155 | Phosphorylation | Uniprot | |
| S1158 | Phosphorylation | Uniprot | |
| S1234 | Phosphorylation | Uniprot | |
| S1324 | Phosphorylation | Uniprot | |
| S1347 | Phosphorylation | Uniprot | |
| S1353 | Phosphorylation | Uniprot | |
| T1366 | Phosphorylation | Uniprot | |
| T1368 | Phosphorylation | Uniprot | |
| Y1369 | Phosphorylation | Uniprot | |
| S1398 | Phosphorylation | Uniprot | |
| S1420 | Phosphorylation | Uniprot | |
| S1421 | Phosphorylation | Uniprot | |
| K1427 | Acetylation | Uniprot | |
| T1440 | Phosphorylation | Uniprot | |
| K1447 | Ubiquitination | Uniprot | |
| K1448 | Ubiquitination | Uniprot | |
| S1480 | Phosphorylation | Uniprot | |
| Y1493 | Phosphorylation | Uniprot | |
| T1494 | Phosphorylation | Uniprot | |
| T1497 | Phosphorylation | Uniprot | |
| S1500 | Phosphorylation | Uniprot | |
| K1505 | Acetylation | Uniprot | |
| T1506 | Phosphorylation | Uniprot | |
| K1524 | Acetylation | Uniprot | |
| S1534 | Phosphorylation | Uniprot | |
| S1539 | Phosphorylation | Uniprot | |
| S1540 | Phosphorylation | Uniprot | |
| S1546 | Phosphorylation | Uniprot | |
| S1555 | Phosphorylation | Uniprot | |
| S1562 | Phosphorylation | Uniprot | |
| S1564 | Phosphorylation | Uniprot | |
| S1588 | Phosphorylation | Uniprot | |
| T1590 | Phosphorylation | Uniprot | |
| S1591 | Phosphorylation | Uniprot | |
| T1592 | Phosphorylation | Uniprot | |
| T1594 | Phosphorylation | Uniprot | |
| T1595 | Phosphorylation | Uniprot | |
| S1598 | Phosphorylation | Uniprot | |
| T1599 | Phosphorylation | Uniprot | |
| T1602 | Phosphorylation | Uniprot | |
| T1605 | Phosphorylation | Uniprot | |
| S1608 | Phosphorylation | Uniprot | |
| Y1609 | Phosphorylation | Uniprot | |
| S1611 | Phosphorylation | Uniprot | |
| T1613 | Phosphorylation | Uniprot | |
| T1616 | Phosphorylation | Uniprot | |
| T1619 | Phosphorylation | Uniprot | |
| S1621 | Phosphorylation | Uniprot | |
| Y1622 | Phosphorylation | Uniprot | |
| T1628 | Phosphorylation | Uniprot | |
| T1631 | Phosphorylation | Uniprot | |
| S1634 | Phosphorylation | Uniprot | |
| S1640 | Phosphorylation | Uniprot | |
| T1649 | Phosphorylation | Uniprot | |
| S1653 | Phosphorylation | Uniprot | |
| T1656 | Phosphorylation | Uniprot | |
| S1675 | Phosphorylation | Uniprot | |
| S1679 | Phosphorylation | P17612 (PRKACA) , Q9P0L2 (MARK1) | Uniprot |
| T1680 | Phosphorylation | Uniprot | |
| S1702 | Phosphorylation | Uniprot | |
| S1706 | Phosphorylation | Uniprot | |
| S1710 | Phosphorylation | Q9P0L2 (MARK1) , P17612 (PRKACA) | Uniprot |
| S1727 | Phosphorylation | Uniprot | |
| S1742 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T1780 | Phosphorylation | Uniprot | |
| S1782 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S1786 | Phosphorylation | Uniprot | |
| S1787 | Phosphorylation | Uniprot | |
| S1790 | Phosphorylation | Uniprot | |
| S1795 | Phosphorylation | Uniprot | |
| S1798 | Phosphorylation | Uniprot | |
| S1799 | Phosphorylation | Uniprot | |
| S1800 | Phosphorylation | Uniprot | |
| S1802 | Phosphorylation | Uniprot | |
| S1808 | Phosphorylation | Uniprot | |
| T1813 | Phosphorylation | Uniprot | |
| T1819 | Phosphorylation | Uniprot |
研究背景
機能:
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
PTMs:
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
細胞の位置付け:
Cytoplasm>Cytoskeleton. Cell projection>Dendrite.
サブユニット構造:
Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites.
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