AFfirm™ COPG Mouse Monoclonal Antibody - #BF8646
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
Coat protein gamma cop; Coatomer protein complex, subunit gamma 1; coatomer protein complex, subunit gamma; Coatomer subunit gamma; Coatomer subunit gamma-1; COP; COPG1; COPG1_HUMAN; FLJ21068; Gamma coat protein; Gamma COP; Gamma-1-coat protein; Gamma-1-COP;
免疫原
- Q9Y678 COPG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLKKFDKKDEESGGGSNPFQHLEKSAVLQEARVFNETPINPRKCAHILTKILYLINQGEHLGTTEATEAFFAMTKLFQSNDPTLRRMCYLTIKEMSCIAEDVIIVTSSLTKDMTGKEDNYRGPAVRALCQITDSTMLQAIERYMKQAIVDKVPSVSSSALVSSLHLLKCSFDVVKRWVNEAQEAASSDNIMVQYHALGLLYHVRKNDRLAVNKMISKVTRHGLKSPFAYCMMIRVASKQLEEEDGSRDSPLFDFIESCLRNKHEMVVYEAASAIVNLPGCSAKELAPAVSVLQLFCSSPKAALRYAAVRTLNKVAMKHPSAVTACNLDLENLVTDSNRSIATLAITTLLKTGSESSIDRLMKQISSFMSEISDEFKVVVVQAISALCQKYPRKHAVLMNFLFTMLREEGGFEYKRAIVDCIISIIEENSESKETGLSHLCEFIEDCEFTVLATRILHLLGQEGPKTTNPSKYIRFIYNRVVLEHEEVRAGAVSALAKFGAQNEEMLPSILVLLKRCVMDDDNEVRDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLERALQQYTLEPSEKPFDLKSVPLATAPMAEQRTESTPITAVKQPEKVAATRQEIFQEQLAAVPEFRGLGPLFKSSPEPVALTESETEYVIRCTKHTFTNHMVFQFDCTNTLNDQTLENVTVQMEPTEAYEVLCYVPARSLPYNQPGTCYTLVALPKEDPTAVACTFSCMMKFTVKDCDPTTGETDDEGYEDEYVLEDLEVTVADHIQKVMKLNFEAAWDEVGDEFEKEETFTLSTIKTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDILVRSRLLLLDTVTMQVTARSLEELPVDIILASVG
PTMs - Q9Y678 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| K145 | Methylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| R304 | Methylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S356 | Phosphorylation | Uniprot | |
| R359 | Methylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| R488 | Methylation | Uniprot | |
| K514 | Ubiquitination | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| S578 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| T597 | Phosphorylation | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| T640 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| Y646 | Phosphorylation | Uniprot | |
| T718 | Phosphorylation | Uniprot | |
| T723 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| T742 | Phosphorylation | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| K818 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot |
研究背景
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).
Cytoplasm. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>COPI-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side.
Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Predominantly located in the cis-Golgi apparatus.
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Interacts with ZNF289/ARFGAP2 through its C-terminal appendage domain. Interacts with EGFR upon EGF treatment; interaction is essential for regulation of EGF-dependent nuclear transport of EGFR by retrograde trafficking from the Golgi to the ER. Interacts with COPB1. Interacts with TMED10 (via C-terminus). Interacts with TMED2, TMED3, TMED7 and TMED9.
Belongs to the COPG family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.