GLUT4 Antibody - #BF1001

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製品: GLUT4 Antibody
カタログ: BF1001
タンパク質の説明: Mouse monoclonal antibody to GLUT4
アプリケーション: WB IHC IF/ICC ELISA FACS
反応性: Human, Mouse
分子量: 54.8kDa; 55kD(Calculated).
ユニプロット: P14672
RRID: AB_2835383

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説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Monoclonal [AFB3365]
特異性:
GLUT4.
RRID:
AB_2835383
引用形式: Affinity Biosciences Cat# BF1001, RRID:AB_2835383.
コンジュゲート:
Unconjugated.
精製:
affinity purification.
保存:
Store at -20°C. Stable for one year from the date of shipment. 1mg/ml in PBS, pH 7.4. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

insulin-responsive; Glucose transporter GLUT 4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GLUT4; GTR4_HUMAN; Insulin responsive glucose transporter type 4; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2 member 4; Solute carrier family 2, facilitated glucose transporter member 4;

免疫原

免疫原:

Purified recombinant fragment of human SLC2A4 (AA: 224-353 ) expressed in E. Coli.

Uniprot:

P14672(GLUT4_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC2A4(6517)
発現特異性:
P14672 GLUT4_HUMAN:

Skeletal and cardiac muscles; brown and white fat.

タンパク質の説明:
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
タンパク質配列:
MPSGFQQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETWLGRQGPEGPSSIPPGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLVNNVLAVLGGSLMGLANAAASYEMLILGRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILIAQVLGLESLLGTASLWPLLLGLTVLPALLQLVLLPFCPESPRYLYIIQNLEGPARKSLKRLTGWADVSGVLAELKDEKRKLERERPLSLLQLLGSRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFETAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRRTLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQISAAFHRTPSLLEQEVKPSTELEYLGPDEND

PTMs - P14672 基板として

Site PTM Type Enzyme
Y56 Phosphorylation
N57 N-Glycosylation
S274 Phosphorylation O00141 (SGK1)
S281 Phosphorylation
T368 Phosphorylation
T486 Phosphorylation
S488 Phosphorylation
Y502 Phosphorylation

研究背景

機能:

Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation. Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell.

PTMs:

Sumoylated.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Endomembrane system>Multi-pass membrane protein. Cytoplasm>Perinuclear region.
Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized (PubMed:8300557). The dileucine internalization motif is critical for intracellular sequestration (PubMed:8300557). Insulin stimulation induces translocation to the cell membrane (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Skeletal and cardiac muscles; brown and white fat.

サブユニット構造:

Interacts with NDUFA9 (By similarity). Binds to DAXX. Interacts via its N-terminus with SRFBP1. Interacts with TRARG1; the interaction is required for proper SLC2A4 recycling after insulin stimulation (By similarity).

タンパク質ファミリー:

The dileucine internalization motif is critical for intracellular sequestration.

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

研究領域

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

参考文献

1). Integrating network analysis and experimental validation to reveal the mechanism of pinocembrin in alleviating high glucose and free fatty acid-induced lipid accumulation in HepG2 cells. Journal of Functional Foods, 2023 [IF=5.6]
2). Esculin ameliorates obesity-induced insulin resistance by improving adipose tissue remodeling and activating the IRS1/PI3K/AKT/GLUT4 pathway. Journal of ethnopharmacology, 2024 (PubMed: 37778516) [IF=5.4]
3). Silencing of ANGPTL8 Alleviates Insulin Resistance in Trophoblast Cells. Frontiers in Endocrinology, 2021 (PubMed: 34163433) [IF=5.2]

Application: WB    Species: Mice    Sample: placenta tissues

Figure 1 Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice. (A) The mice were treated as described in the chart. (B) The body weight of mice in normal fat diet (NFD) and high fat diet (HFD) groups. (C) Oral glucose tolerance test (OGTT) was performed at gestational day (GD)0.5, 11.5 and 16.5. (D, E) Fasting blood glucose and insulin levels were measured at GD18.5. (F) Homeostasis model assessment insulin resistance (HOMA-IR) was calculated as follow: HOMA-IR= blood glucose (mM)×blood insulin (mU/l)/22.5. (G) The contents of triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) in serum were detected. (H) HE staining was performed to detect the pathological changes in labyrinth zone of placenta tissues. (I) Periodic acid Schiff (PAS) staining was carried out to detect the glycogen accumulation in labyrinth zone of placenta tissues. (J) Western blot was used to determine the levels of insulin signaling related molecules, p-IRβ(Tyr1361), IRβ, p-IRS-1(Ser307), p-IRS-1(Tyr896), IRS-1, p-Akt and Akt in placenta tissues. (K) The expression levels of glucose transporter 1 (GLUT1) and GLUT4 in placenta tissues. (L) The serum level of ANGPTL8 in mice. (M, N) The mRNA and protein levels of ANGPTL8 in placenta tissues. (the scale bar represents 100 μm; **p < 0.01, ***p < 0.001 vs. NFD).

Application: WB    Species: mouse    Sample: placenta

FIGURE 1 | Angiopoietin like-8 (ANGPTL8) was increased in serum and placenta tissues of gestational diabetes mellitus (GDM) mice. (K) The expression levels of glucose transporter 1 (GLUT1) and GLUT4 in placenta tissues.

Application: IF/ICC    Species: human    Sample: HTR-8/SVneo cell

FIGURE 2 | Silencing of ANGPTL8 inhibited IR in trophoblast cells.(F, G) Immunofluorescent staining was used to detect the expression and distribution of GLUT1 and GLUT4 in HTR-8/SVneo cells. (the scale bar represents 50 mm; *p < 0.05, ***p < 0.001,ns, no significance).
4). Agomelatine Exerts an Anti-inflammatory Effect by Inhibiting Microglial Activation Through TLR4/NLRP3 Pathway in pMCAO Rats. NEUROTOXICITY RESEARCH, 2022 (PubMed: 34843079) [IF=3.7]
5). Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions. Iranian Journal of Basic Medical Sciences, 2023 [IF=2.2]

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