AFfirm™ CHCHD3 Mouse Monoclonal Antibody - #BF8828
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
CHCH3_HUMAN; CHCHD 3; Chchd3; Coiled coil helix coiled coil helix domain containing 3; Coiled coil helix coiled coil helix domain containing protein 3 mitochondrial; Coiled-coil-helix-coiled-coil-helix domain-containing protein 3; FLJ20420; MINOS3; mitochondrial; Mitochondrial inner membrane organizing system 3; PPP1R22; Protein phosphatase 1 regulatory subunit 22;
免疫原
Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
- Q9NX63 MIC19_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG
PTMs - Q9NX63 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| T11 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S29 | Phosphorylation | Uniprot | |
| R35 | Methylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K173 | Acetylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| Y177 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot |
研究背景
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.
Mitochondrion inner membrane>Lipid-anchor>Intermembrane side. Cytoplasm. Nucleus. Mitochondrion.
Detected at low levels in brain, placenta, lung, liver, kidney and pancreas with increased levels in heart and skeletal muscle. Higher expression in primary lung cancers than in normal lung tissue.
Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and MICOS13/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with HSPA1A/HSPA1B and OPA1, preferentially with the soluble OPA1 form (By similarity). Interacts with IMMT/MIC60.
Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic19 subfamily.
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