製品: YTHDF2 Mouse Monoclonal Antibody
カタログ: BF8840
タンパク質の説明: Mouse monoclonal antibody to YTHDF2
アプリケーション: WB
反応性: Human
予測: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 62 kDa; 62kD(Calculated).
ユニプロット: Q9Y5A9

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm8840]
特異性:
YTHDF2 Mouse Monoclonal Antibody detects endogenous levels of total YTHDF2.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

9430020E02Rik; CAHL; CLL associated antigen KW 14; CLL-associated antigen KW-14; HGRG8; High glucose regulated protein 8; High-glucose-regulated protein 8; NY REN 2; Renal carcinoma antigen NY REN 2; Renal carcinoma antigen NY-REN-2; YTH domain family 2; YTH domain family member 2; YTH domain family protein 2; YTH N6 methyladenosine RNA binding protein 2; YTHD2_HUMAN; YTHDF 2; YTHDF2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSASSLLEQRPKGQGNKVQNGSVHQKDGLNDDDFEPYLSPQARPNNAYTAMSDSYLPSYYSPSIGFSYSLGEAAWSTGGDTAMPYLTSYGQLSNGEPHFLPDAMFGQPGALGSTPFLGQHGFNFFPSGIDFSAWGNNSSQGQSTQSSGYSSNYAYAPSSLGGAMIDGQSAFANETLNKAPGMNTIDQGMAALKLGSTEVASNVPKVVGSAVGSGSITSNIVASNSLPPATIAPPKPASWADIASKPAKQQPKLKTKNGIAGSSLPPPPIKHNMDIGTWDNKGPVAKAPSQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQPTRWVAPRNRGSGFGHNGVDGNGVGQSQAGSGSTPSEPHPVLEKLRSINNYNPKDFDWNLKHGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSMNGKGPVYLLFSVNGSGHFCGVAEMKSAVDYNTCAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLEKAKQVLKIIASYKHTTSIFDDFSHYEKRQEEEESVKKERQGRGK

PTMs - Q9Y5A9 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S4 Phosphorylation
S5 Phosphorylation
K12 Ubiquitination
K17 Ubiquitination
K26 Ubiquitination
Y37 Phosphorylation
S39 Phosphorylation
K193 Ubiquitination
S196 Phosphorylation
S238 Phosphorylation
K245 Acetylation
T277 Phosphorylation
K281 Ubiquitination
R350 Methylation
R357 Methylation
S359 Phosphorylation
S380 Phosphorylation
T381 Phosphorylation
S383 Phosphorylation
K391 Ubiquitination
R393 Methylation
S394 Phosphorylation
K401 Acetylation
K401 Ubiquitination
K408 Ubiquitination
K416 Ubiquitination
S419 Phosphorylation
K428 Ubiquitination
Y446 Phosphorylation
K490 Ubiquitination
K521 Ubiquitination
K536 Ubiquitination
K548 Ubiquitination
S558 Phosphorylation
Y560 Phosphorylation
K562 Ubiquitination
S569 Phosphorylation

研究背景

機能:

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT and ribonuclease P/MRP complexes, depending on the context. M6A-containing mRNAs containing a binding site for RIDA/HRSP12 (5'-GGUUC-3') are preferentially degraded by endoribonucleolytic cleavage: cooperative binding of RIDA/HRSP12 and YTHDF2 to transcripts leads to recruitment of the ribonuclease P/MRP complex. Other m6A-containing mRNAs undergo deadenylation via direct interaction between YTHDF2 and CNOT1, leading to recruitment of the CCR4-NOT and subsequent deadenylation of m6A-containing mRNAs. Required maternally to regulate oocyte maturation: probably acts by binding to m6A-containing mRNAs, thereby regulating maternal transcript dosage during oocyte maturation, which is essential for the competence of oocytes to sustain early zygotic development (By similarity). Also involved in hematopoietic stem cells specification by binding to m6A-containing mRNAs, leading to promote their degradation. Also acts as a regulator of neural development by promoting m6A-dependent degradation of neural development-related mRNA targets (By similarity). Regulates circadian regulation of hepatic lipid metabolism: acts by promoting m6A-dependent degradation of PPARA transcripts. Regulates the innate immune response to infection by inhibiting the type I interferon response: acts by binding to m6A-containing IFNB transcripts and promoting their degradation. Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation.

(Microbial infection) Promotes viral gene expression and replication of polyomavirus SV40: acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.

(Microbial infection) Promotes viral gene expression and virion production of kaposis sarcoma-associated herpesvirus (KSHV) at some stage of the KSHV life cycle (in iSLK.219 and iSLK.BAC16 cells). Acts by binding to N6-methyladenosine (m6A)-containing viral RNAs.

細胞の位置付け:

Cytoplasm>Cytosol. Nucleus. Cytoplasm>P-body.
Note: Localizes to the cytosol and relocates to the nucleus following heat shock stress (PubMed:26458103).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Interacts with YTHDF3. Interacts with CNOT1; interaction is direct and promotes recruitment of the CCR4-NOT complex. Interacts with RIDA/HRSP12; interaction leads to recruitment of the ribonuclease P/MRP complex.

タンパク質ファミリー:

Belongs to the YTHDF2 family.

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