AFfirm™ NASP Mouse Monoclonal Antibody - #BF8851
製品説明
ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human
クローナリティ:
Monoclonal [AFfirm8851]
特異性:
NASP Mouse Monoclonal Antibody detects endogenous levels of total NASP.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
5033430J04Rik; AI131596; AI317140; D4Ertd767e; DKFZp547F162; Epcs32; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; MGC93869; NASP; NASP_HUMAN; nuclear autoantigenic sperm protein (histone binding); Nuclear autoantigenic sperm protein; PRO1999; RP23 233B9.9;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
タンパク質配列:
- P49321 NASP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEGEKTEDESLVENNDNIDEEAREELREQVYDAMGEKEEAKKTEDKSLAKPETDKEQDSEMEKGGREDMDISKSAEEPQEKVDLTLDWLTETSEEAKGGAAPEGPNEAEVTSGKPEQEVPDAEEEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEEQPVVTLEKQGTAVEVEAESLDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAEESPEVTTEAAEASAVEAGSEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIERLTETKDGSGLEEKVRAKLVPSQEETKLSVEESEAAGDGVDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGSEEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAGATVESTAC
PTMs - P49321 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K20 | Acetylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T123 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| R140 | Methylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| C254 | S-Nitrosylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| T291 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K417 | Acetylation | Uniprot | |
| K417 | Ubiquitination | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| T448 | Phosphorylation | Uniprot | |
| K450 | Acetylation | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| K455 | Acetylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| T490 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| Y540 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| Y575 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| R617 | Methylation | Uniprot | |
| K626 | Ubiquitination | Uniprot | |
| K636 | Sumoylation | Uniprot | |
| K643 | Acetylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| T683 | Phosphorylation | Uniprot | |
| S689 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S696 | Phosphorylation | Uniprot | |
| K698 | Acetylation | Uniprot | |
| K698 | Ubiquitination | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| K721 | Methylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| K733 | Acetylation | Uniprot | |
| K734 | Acetylation | Uniprot | |
| K736 | Acetylation | Uniprot | |
| K736 | Sumoylation | Uniprot | |
| S745 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S756 | Phosphorylation | Uniprot | |
| C788 | S-Nitrosylation | Uniprot |
研究背景
機能:
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
細胞の位置付け:
Cytoplasm. Nucleus.
組織特異性:
Isoform 1 is testis- and sperm-specific.
サブユニット構造:
Binds to linker H1 histones but not to core histones (By similarity). Interacts with histones H2A, H2B, H3 and H4. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).
タンパク質ファミリー:
Belongs to the NASP family.
Restrictive clause
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For Research Use Only.
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