AFfirm™ EPHA1 Mouse Monoclonal Antibody - #BF8866
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
EPH; EPH receptor A1; EPH tyrosine kinase 1; EPH tyrosine kinase; Epha1; EPHA1_HUMAN; Ephrin receptor Eph A1; Ephrin type A receptor 1; Ephrin type-A receptor 1; EPHT 1; EPHT; EPHT1; Erythropoietin producing hepatoma amplified sequence; Erythropoietin-producing hepatoma receptor; Esk; hEpha1; MGC163163; Oncogene EPH; Soluble EPHA1 variant 1; Soluble EPHA1 variant 2; Tyrosine protein kinase receptor EPH; Tyrosine-protein kinase receptor EPH;
免疫原
- P21709 EPHA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERRWPLGLGLVLLLCAPLPPGARAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCPMQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLVSGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNGLAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVACPSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSLRWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVEAQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDEERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAVIFGLLLGAALLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVIGEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMIITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRMTLRLPSLSGSDGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD
PTMs - P21709 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T33 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| Y599 | Phosphorylation | Uniprot | |
| Y605 | Phosphorylation | Uniprot | |
| T780 | Phosphorylation | Uniprot | |
| Y781 | Phosphorylation | Uniprot | |
| T783 | Phosphorylation | Uniprot | |
| K787 | Ubiquitination | Uniprot | |
| T805 | Phosphorylation | Uniprot | |
| S811 | Phosphorylation | Uniprot | |
| T892 | Phosphorylation | Uniprot | |
| S906 | Phosphorylation | Uniprot | |
| S908 | Phosphorylation | Uniprot | |
| S910 | Phosphorylation | Uniprot | |
| S919 | Phosphorylation | Uniprot |
研究背景
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated.
Cell membrane>Single-pass type I membrane protein.
Overexpressed in several carcinomas.
Homodimer. Forms a signaling complex with LCK; PTK2B/PYK2 and PI3-kinase upon activation by EFNA1; regulates T-lymphocytes migration. Interacts (via SAM domain) with ILK (via ANK repeats); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Interacts (kinase activity-dependent) with PTK2/FAK1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
研究領域
· Organismal Systems > Development > Axon guidance. (View pathway)
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