AFfirm™ PTP alpha Mouse Monoclonal Antibody - #BF8879
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
HEPTP; HLPR; HPTP alpha; HPTPA; HPTPalpha; Leukocyte common antigen related peptide; LRP; Protein tyrosine phosphatase alpha; Protein tyrosine phosphatase receptor type A; Protein tyrosine phosphatase receptor type alpha polypeptide; Protein tyrosine phosphate; Protein-tyrosine phosphatase alpha; PTPA; PTPalpha; PTPase alpha; PTPLCA related phosphatase; Ptpra; PTPRA_HUMAN; PTPRL 2; PTPRL2; PTRA; R PTP alpha; R-PTP-alpha; Receptor type tyrosine protein phosphatase alpha; Receptor-type tyrosine-protein phosphatase alpha; RPTPA; Tyrosine phosphatase alpha;
免疫原
- P18433 PTPRA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSWFILVLLGSGLICVSANNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNITLGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSGNSDSKDRRDETPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYPPLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEGVPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGCWTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKACNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALLEHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIPYEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERGQEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGMISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLEQYEFCYKVVQEYIDAFSDYANFK
PTMs - P18433 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S18 | Phosphorylation | Uniprot | |
| S187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| S204 | Phosphorylation | Q9UQM7 (CAMK2A) , Q05655 (PRKCD) | Uniprot |
| S211 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| T214 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| Y522 | Phosphorylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| T552 | Phosphorylation | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| K765 | Acetylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| Y791 | Phosphorylation | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| Y798 | Phosphorylation | P12931 (SRC) | Uniprot |
研究背景
Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (By similarity). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (By similarity).
Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.
Cell membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion.
Note: Localizes to focal adhesion sites following integrin engagement.
Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain), and SRC. Within the complex, interacts (when phosphorylated on Tyr-798) with BCAR3 (via SH2 domain). Interacts with GRB2.
Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.
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