AFfirm™ PTRF Mouse Monoclonal Antibody - #BF8886
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
2310075E07Rik; AW546441; Cavin 1; Cavin; Cavin-1; CAVIN1; CGL4; FKSG13; FLJ90031; MGC118550; OTTMUSP00000002049; Polymerase I and transcript release factor; Ptrf; PTRF_HUMAN; RNA polymerase I and transcript release factor; RP23-279L23.6; TTF I interacting peptide 12;
免疫原
- Q6NZI2 CAVN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDPTLYIVERPLPGYPDAEAPEPSSAGAQAAEEPSGAGSEELIKSDQVNGVLVLSLLDKIIGAVDQIQLTQAQLEERQAEMEGAVQSIQGELSKLGKAHATTSNTVSKLLEKVRKVSVNVKTVRGSLERQAGQIKKLEVNEAELLRRRNFKVMIYQDEVKLPAKLSISKSLKESEALPEKEGEELGEGERPEEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD
PTMs - Q6NZI2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| T103 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| K116 | Sumoylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| Y156 | Phosphorylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K161 | Sumoylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K165 | Acetylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| K170 | Sumoylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K173 | Sumoylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| T302 | Phosphorylation | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K317 | Ubiquitination | Uniprot | |
| K326 | Acetylation | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K327 | Acetylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot |
研究背景
Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues. Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).
Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity. Phosphorylation at Tyr-156 is essential for its functionin the regulation of ribosomal transcriptional activity.
Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.
Monoubiquitinated.
Membrane>Caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm>Cytosol. Mitochondrion. Nucleus.
Note: Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity).
Component of the CAVIN complex composed of CAVIN1, CAVIN2, CAVIN3 and CAVIN4. Homotrimer (By similarity). Interacts with TTF1. Interacts with RNA polymerase I. Binds the 3' end of pre-rRNA. Interacts with transcription factor ZNF148 (By similarity). Interacts with LIPE in the adipocyte cytoplasm. Interacts with CAV1 and CAVIN3 (By similarity). Interacts with CAVIN2. Interacts with CAVIN4 and CAV3.
The leucine-zipper domain 1 is essential for its localization in the caveolae.
Belongs to the CAVIN family.
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