製品: PTRF Mouse Monoclonal Antibody
カタログ: BF8886
タンパク質の説明: Mouse monoclonal antibody to PTRF
アプリケーション: WB
反応性: Human, Mouse
予測: Rat, Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 43 kDa; 43kD(Calculated).
ユニプロット: Q6NZI2

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製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Monoclonal [AFfirm8886]
特異性:
PTRF Mouse Monoclonal Antibody detects endogenous levels of total PTRF.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

2310075E07Rik; AW546441; Cavin 1; Cavin; Cavin-1; CAVIN1; CGL4; FKSG13; FLJ90031; MGC118550; OTTMUSP00000002049; Polymerase I and transcript release factor; Ptrf; PTRF_HUMAN; RNA polymerase I and transcript release factor; RP23-279L23.6; TTF I interacting peptide 12;

免疫原

免疫原:

A synthesized peptide derived from human PTRF, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MEDPTLYIVERPLPGYPDAEAPEPSSAGAQAAEEPSGAGSEELIKSDQVNGVLVLSLLDKIIGAVDQIQLTQAQLEERQAEMEGAVQSIQGELSKLGKAHATTSNTVSKLLEKVRKVSVNVKTVRGSLERQAGQIKKLEVNEAELLRRRNFKVMIYQDEVKLPAKLSISKSLKESEALPEKEGEELGEGERPEEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD

研究背景

機能:

Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues. Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).

PTMs:

Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity. Phosphorylation at Tyr-156 is essential for its functionin the regulation of ribosomal transcriptional activity.

Five truncated forms are found in the caveolae. These are thought to be the result of proteolysis and may be phosphorylation-dependent.

Monoubiquitinated.

細胞の位置付け:

Membrane>Caveola. Cell membrane. Microsome. Endoplasmic reticulum. Cytoplasm>Cytosol. Mitochondrion. Nucleus.
Note: Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Colocalizes with CAV1 in lipid rafts in adipocytes. Localizes in the caveolae in a caveolin-dependent manner (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The leucine-zipper domain 1 is essential for its localization in the caveolae.

Belongs to the CAVIN family.

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