AFfirm™ TDG Mouse Monoclonal Antibody - #BF8968
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
折りたたみ／展開
C JUN leucine zipper interactive protein; C JUN leucine zipper interactive protein; C-JUN leucine zipper interactive protein JZA-3; E130317C12Rik; EC 3.2.2.29; G/T mismatch specific thymine DNA glycosylase; G/T mismatch specific thymine DNA glycosylase; G/T mismatch-specific thymine DNA glycosylase; JZA 3; Jza1; T:G mismatch thymine glycosylase; Tdg; TDG_HUMAN; Thymine DNA glycosylase; Thymine-DNA glycosylase;
免疫原
- Q13569 TDG_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAENAGSYSLQQAQAFYTFPFQQLMAEAPNMAVVNEQQMPEEVPAPAPAQEPVQEAPKGRKRKPRTTEPKQPVEPKKPVESKKSGKSAKSKEKQEKITDTFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYVMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNMDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENPCSSEPCGFSSNGLIESVELRGESAFSGIPNGQWMTQSFTDQIPSFSNHCGTQEQEEESHA
PTMs - Q13569 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K71 | Ubiquitination | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| K83 | Acetylation | Uniprot | |
| K84 | Acetylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K94 | Acetylation | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| K248 | Sumoylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K330 | Sumoylation | Uniprot |
研究背景
DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
Nucleus.
Homodimer. Interacts with AICDA and GADD45A.
Belongs to the uracil-DNA glycosylase (UDG) superfamily. TDG/mug family.
研究領域
· Genetic Information Processing > Replication and repair > Base excision repair.
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