AFfirm™ PGP9.5 Mouse Monoclonal Antibody - #BF8987

製品: PGP9.5 Mouse Monoclonal Antibody
カタログ: BF8987
タンパク質の説明: Mouse monoclonal antibody to PGP9.5
アプリケーション: WB
反応性: Human
予測: Mouse, Rat, Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 25 kDa; 25kD(Calculated).
ユニプロット: P09936

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説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm8987]
特異性:
PGP9.5 Mouse Monoclonal Antibody detects endogenous levels of total PGP9.5.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Epididymis luminal protein 117; Epididymis secretory protein Li 53; HEL 117; HEL S 53; NDGOA; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; Park 5; PARK5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase; Ubiquitin C terminal hydrolase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH-L1; UCHL1; UCHL1_HUMAN;

免疫原

免疫原:
Uniprot:

P09936(UCHL1_HUMAN) >>Visit HPA database.

遺伝子(ID):
UCHL1(7345)
発現特異性:
P09936 UCHL1_HUMAN:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

タンパク質の説明:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes.
タンパク質配列:
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

PTMs - P09936 基板として

Site PTM Type Enzyme
K4 Acetylation
K4 Ubiquitination
K15 Ubiquitination
K64 Ubiquitination
K65 Ubiquitination
K71 Ubiquitination
K78 Ubiquitination
K83 Ubiquitination
K105 Ubiquitination
K115 Ubiquitination
S119 Phosphorylation
K123 Ubiquitination
C132 S-Nitrosylation
K135 Ubiquitination
C152 S-Nitrosylation
K157 Ubiquitination
S188 Phosphorylation
S189 Phosphorylation
T192 Phosphorylation
K195 Acetylation
K195 Ubiquitination
C220 S-Nitrosylation
K221 Ubiquitination

研究背景

機能:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity.

PTMs:

O-glycosylated.

細胞の位置付け:

Cytoplasm. Endoplasmic reticulum membrane>Lipid-anchor.
Note: About 30% of total UCHL1 is associated with membranes in brain.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

サブユニット構造:

Monomer. Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.

タンパク質ファミリー:

Belongs to the peptidase C12 family.

研究領域

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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