AFfirm™ Desmin Mouse Monoclonal Antibody - #BF8994

製品: Desmin Mouse Monoclonal Antibody
カタログ: BF8994
タンパク質の説明: Mouse monoclonal antibody to Desmin
アプリケーション: WB
反応性: Human
予測: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Monkey, Chicken, Xenopus
分子量: 52 kDa; 54kD(Calculated).
ユニプロット: P17661

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Mouse
アプリケーション:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Monoclonal [AFfirm8994]
特異性:
Desmin Mouse Monoclonal Antibody detects endogenous levels of total Desmin.
コンジュゲート:
Unconjugated.
精製:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CMD1I; CSM1; CSM2; DES; DESM_HUMAN; Desmin; FLJ12025; FLJ39719; FLJ41013; FLJ41793; Intermediate filament protein; OTTHUMP00000064865;

免疫原

免疫原:
Uniprot:

P17661(DESM_HUMAN) >>Visit HPA database.

遺伝子(ID):
DES(1674)
タンパク質の説明:
Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
タンパク質配列:
MSQAYSSSQRVSSYRRTFGGAPGFPLGSPLSSPVFPRAGFGSKGSSSSVTSRVYQVSRTSGGAGGLGSLRASRLGTTRTPSSYGAGELLDFSLADAVNQEFLTTRTNEKVELQELNDRFANYIEKVRFLEQQNAALAAEVNRLKGREPTRVAELYEEELRELRRQVEVLTNQRARVDVERDNLLDDLQRLKAKLQEEIQLKEEAENNLAAFRADVDAATLARIDLERRIESLNEEIAFLKKVHEEEIRELQAQLQEQQVQVEMDMSKPDLTAALRDIRAQYETIAAKNISEAEEWYKSKVSDLTQAANKNNDALRQAKQEMMEYRHQIQSYTCEIDALKGTNDSLMRQMRELEDRFASEASGYQDNIARLEEEIRHLKDEMARHLREYQDLLNVKMALDVEIATYRKLLEGEESRINLPIQTYSALNFRETSPEQRGSEVHTKKTVMIKTIETRDGEVVSEATQQQHEVL

PTMs - P17661 基板として

Site PTM Type Enzyme
Y5 Phosphorylation
S6 Phosphorylation
S7 Phosphorylation
S8 Phosphorylation
S12 Phosphorylation Q96GD4 (AURKB) , P17612 (PRKACA) , Q13464 (ROCK1)
S13 Phosphorylation
T17 Phosphorylation P17612 (PRKACA) , Q13464 (ROCK1) , Q96GD4 (AURKB)
S28 Phosphorylation
S31 Phosphorylation
S32 Phosphorylation P06493 (CDK1)
S42 Phosphorylation
K43 Methylation
S45 Phosphorylation P17612 (PRKACA)
Y54 Phosphorylation
S57 Phosphorylation
T59 Phosphorylation
S60 Phosphorylation Q13464 (ROCK1) , Q96GD4 (AURKB) , P17612 (PRKACA)
S68 Phosphorylation
S72 Phosphorylation
T76 Phosphorylation Q13464 (ROCK1) , P17612 (PRKACA)
T77 Phosphorylation Q13464 (ROCK1)
T79 Phosphorylation
S81 Phosphorylation
S82 Phosphorylation
Y83 Phosphorylation
T106 Phosphorylation
K109 Acetylation
K109 Ubiquitination
Y122 Phosphorylation
Y155 Phosphorylation
T170 Phosphorylation
S231 Phosphorylation
K240 Acetylation
K241 Acetylation
Y281 Phosphorylation
Y296 Phosphorylation
S301 Phosphorylation
R315 Methylation
Y324 Phosphorylation
S330 Phosphorylation
Y331 Phosphorylation
T332 Phosphorylation
K339 Acetylation
S358 Phosphorylation
Y363 Phosphorylation
R386 Methylation
Y388 Phosphorylation
K407 Acetylation
K407 Sumoylation
K407 Ubiquitination
S414 Phosphorylation
Y423 Phosphorylation
S424 Phosphorylation
S432 Phosphorylation
T450 Phosphorylation

研究背景

機能:

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

PTMs:

ADP-ribosylation prevents ability to form intermediate filaments.

Phosphorylation at Ser-7, Ser-28 and Ser-32 by CDK1, phosphorylation at Ser-60 by AURKB and phosphorylation at Thr-76 by ROCK1 contribute to efficient separation of desmin intermediate filaments during mitosis.

細胞の位置付け:

Cytoplasm>Myofibril>Sarcomere>Z line. Cytoplasm. Cell membrane>Sarcolemma. Nucleus.
Note: Localizes in the intercalated disks which occur at the Z line of cardiomyocytes (PubMed:24200904, PubMed:26724190). Localizes in the nucleus exclusively in differentiating cardiac progenitor cells and premature cardiomyocytes (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homopolymer. Interacts with DST (By similarity). Interacts with MTM1. Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament. Interacts with CRYAB. Interacts with NEB (via nebulin repeats 160-164). Interacts (via rod region) with NEBL (via nebulin repeats 1-5).

タンパク質ファミリー:

Belongs to the intermediate filament family.

研究領域

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

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